Article

Mesoporous silica nanoparticles deliver DNA and chemicals into plants. Nat Nanotech 2:295-300

Nature Nanotechnology (Impact Factor: 34.05). 04/2007; 2(5):295-300. DOI: 10.1038/nnano.2007.108

ABSTRACT

Surface-functionalized silica nanoparticles can deliver DNA1, 2, 3, 4, 5, 6, 7, 8 and drugs9, 10, 11, 12, 13, 14, 15 into animal cells and tissues. However, their use in plants is limited by the cell wall present in plant cells. Here we show a honeycomb mesoporous silica nanoparticle (MSN) system with 3-nm pores that can transport DNA and chemicals into isolated plant cells and intact leaves. We loaded the MSN with the gene and its chemical inducer and capped the ends with gold nanoparticles to keep the molecules from leaching out. Uncapping the gold nanoparticles released the chemicals and triggered gene expression in the plants under controlled-release conditions. Further developments such as pore enlargement and multifunctionalization of these MSNs may offer new possibilities in target-specific delivery of proteins, nucleotides and chemicals in plant biotechnology.

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Available from: Francois J Torney
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    • "NPs used for these purposes include calcium phosphate, carbon, silica, gold magnetite, strontium phosphate. Pore enlargement and multifunctionalization of mesoporous silica NPs could facilitate target-specific delivery of proteins, nucleotides and chemicals in plant biotechnology (Torney et al. 2007). effects of NPs on plant photosynthetic characteristics "
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    • "Nanotechnology advancements in nutrition strategies for plants attempt to solve Nitrogen (N) losses via fertilizer application by introducing Multi-wall carbon nanotubes (MWNTs) as a carrier to improve nutrient uptake by plant cells (Torney et al., 2007; Gonzales-Melendi et al., 2008; Serag et al., 2011). Upon successful delivery into the cells, the absorption and utilization of N by the plant can be increased leading to enhanced plant growth. "

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    • "In recent years, a breakthrough has been made as a result of Torney et al. (2007) who were able to control the intracellular release of substances into protoplasts using mesoporous silica nanoparticles. Despite these advances, the delivery of nanoparticles into plant tissues has been limited to methods involving bombardment, a methodology that does not allow massive application of particles in large number of plants, thus being less exploited in crop improvement programs till date (Torney et al. 2007). Copper Bio-mineralisation with some wetland plants that transform copper into metallic nanoparticles at soil-root interface with the help of some endomycorrhizal fungi were reported that could reduce copper toxicity in the contaminated soils (Manceau et al. 2008). "
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