Mesoporous silica nanoparticles deliver DNA and chemicals into plants. Nat Nanotech 2:295-300

Nature Nanotechnology (Impact Factor: 34.05). 04/2007; 2(5):295-300. DOI: 10.1038/nnano.2007.108


Surface-functionalized silica nanoparticles can deliver DNA1, 2, 3, 4, 5, 6, 7, 8 and drugs9, 10, 11, 12, 13, 14, 15 into animal cells and tissues. However, their use in plants is limited by the cell wall present in plant cells. Here we show a honeycomb mesoporous silica nanoparticle (MSN) system with 3-nm pores that can transport DNA and chemicals into isolated plant cells and intact leaves. We loaded the MSN with the gene and its chemical inducer and capped the ends with gold nanoparticles to keep the molecules from leaching out. Uncapping the gold nanoparticles released the chemicals and triggered gene expression in the plants under controlled-release conditions. Further developments such as pore enlargement and multifunctionalization of these MSNs may offer new possibilities in target-specific delivery of proteins, nucleotides and chemicals in plant biotechnology.

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Available from: Francois J Torney
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    • "NPs used for these purposes include calcium phosphate, carbon, silica, gold magnetite, strontium phosphate. Pore enlargement and multifunctionalization of mesoporous silica NPs could facilitate target-specific delivery of proteins, nucleotides and chemicals in plant biotechnology (Torney et al. 2007). effects of NPs on plant photosynthetic characteristics "
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    ABSTRACT: Nanoparticles (NPs) are literally and figuratively infiltrating all fields of biological research. They are sophisticated tools that can be customized, either by smart engineering or by the attachment of specific ligands, to match the requirements of a particular task. Through their inherent and functionalized properties they are the basis for new developments while enhancing the efficiency of already existing techniques or rendering methods to be more specific. They provide new approaches for therapeutic applications and brand new platforms for diagnostic processes. In this review we provide an insight into the practical applications of NPs, emphasizing their use in biosensing, bioimaging, biomolecule delivery systems and enzyme immobilization. Since the interest in the interactions of NPs and biological systems is fairly new, we also elaborate on the drawbacks of their practical applications by reporting their potential toxicity in in vitro and in vivo systems.
    Full-text · Article · Oct 2015 · Acta Biologica Szegediensis
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    • "Nanotechnology advancements in nutrition strategies for plants attempt to solve Nitrogen (N) losses via fertilizer application by introducing Multi-wall carbon nanotubes (MWNTs) as a carrier to improve nutrient uptake by plant cells (Torney et al., 2007; Gonzales-Melendi et al., 2008; Serag et al., 2011). Upon successful delivery into the cells, the absorption and utilization of N by the plant can be increased leading to enhanced plant growth. "

    Full-text · Article · Jul 2015 · Procedia - Social and Behavioral Sciences
    • "In recent years, a breakthrough has been made as a result of Torney et al. (2007) who were able to control the intracellular release of substances into protoplasts using mesoporous silica nanoparticles. Despite these advances, the delivery of nanoparticles into plant tissues has been limited to methods involving bombardment, a methodology that does not allow massive application of particles in large number of plants, thus being less exploited in crop improvement programs till date (Torney et al. 2007). Copper Bio-mineralisation with some wetland plants that transform copper into metallic nanoparticles at soil-root interface with the help of some endomycorrhizal fungi were reported that could reduce copper toxicity in the contaminated soils (Manceau et al. 2008). "
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    ABSTRACT: Fertilizers play a pivotal role in improving the productivity across the spectrum of crops. The nutrient use efficiencies of conventional fertilizers hardly exceed 30–35 %, 18–20 %, and 35–40 % for N, P, and K which remained constant for the past several decades. Nano-fertilizers intended to improve the nutrient use efficiencies by exploiting unique properties of nanoparticles. The nano-fertilizers are synthesized by fortifying nutrients singly or in combinations onto the adsorbents with nano-dimension. Both physical (top-down) and chemical (bottom-up) approaches are used to produce nanomaterials, and the targeted nutrients are loaded as it is for cationic nutrients (NH4 +, K+, Ca2+, Mg2+) and after surface modification for anionic nutrients (NO3 −, PO4 2−, SO4 2−). Nano-fertilizers are known to release nutrients slowly and steadily for more than 30 days which may assist in improving the nutrient use efficiency without any associated ill-effects. Since the nano-fertilizers are designed to deliver slowly over a long period of time, the loss of nutrients is substantially reduced vis-a-vis environmental safety. The work done on nano-fertilizers is very limited across the globe, but the reported literature clearly demonstrated that these customized fertilizers have a potential role to play in sustaining farm productivity. This chapter focuses on synthesis and characteristics of macro- and micronutrient carrying nano-fertilizers and their application in achieving balanced crop nutrition
    No preview · Chapter · Apr 2015
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