Formation of artificial lipid bilayers using droplet dielectrophoresis

ArticleinLab on a Chip 8(10):1617-20 · November 2008with5 Reads
Impact Factor: 6.12 · DOI: 10.1039/b807374k · Source: PubMed

    Abstract

    We describe the formation of artificial bilayer lipid membranes (BLMs) by the controlled, electrical manipulation of aqueous droplets immersed in a lipid-alkane solution. Droplet movement was generated using dielectrophoresis on planar microelectrodes covered in a thin insulator. Droplets, surrounded by lipid monolayers, were brought into contact and spontaneously formed a BLM. The method produced BLMs suitable for single-channel recording of membrane protein activity and the technique can be extended to create programmable BLM arrays and networks.