Researchers find no link between XMRV and chronic fatigue syndrome

ArticleinBMJ (online) 345(sep19 2):e6337 · September 2012
DOI: 10.1136/bmj.e6331 · Source: PubMed
    • "The results were widely used to link the measles, mumps, and Rubella (MMR) vaccine to the occurrence of autism in children and provide a graphical example of the damage an improperly conducted and inadequately published scientific study can do. Other, less egregious examples of the problems that arise from poor experimental practice include the controversy surrounding the lack of association of xenotropic murine leukaemia virus-related virus (XMRV) in prostate cancer [3] and chronic fatigue syndrome [4] and the retraction of a paper describing the migration of mRNA to initiate flowering, which was a " breakthrough of the year " [5]. In response, a growing consensus has been developing around the need to improve the transparency of reporting of relevant experimental detail to include every aspect important to the qPCR assay itself as well as issues relating to pre-and post-assay parameters . "
    [Show abstract] [Hide abstract] ABSTRACT: The MIQE (minimum information for the publication of quantitative real-time PCR) guidelines were published in 2009 with the twin aims of providing a blueprint for good real-time quantitative polymerase chain reaction (qPCR) assay design and encouraging the comprehensive reporting of qPCR protocols. It had become increasingly clear that variable pre-assay conditions, poor assay design, and incorrect data analysis were leading to the routine publication of data that were often inconsistent, inaccurate, and wrong. The problem was exacerbated by a lack of transparency of reporting, with the details of technical information inadequate for the purpose of assessing the validity of published qPCR data. This had, and continues to have serious implications for basic research, reducing the potential for translating findings into valuable applications and potentially devastating consequences for clinical practice. Today, the rationale underlying the MIQE guidelines has become widely accepted, with more than 2,200 citations by March 2014 and editorials in Nature and related publications acknowledging the enormity of the problem. However, the problem we now face is rather serious: thousands of publications that report suspect data are populating and corrupting the peer-reviewed scientific literature. It will be some time before the many contradictions apparent in every area of the life sciences are corrected.
    Full-text · Article · Apr 2014

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