The mitochondrial citrate transporter, CIC, is essential for mitochondrial homeostasis

Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.
Oncotarget (Impact Factor: 6.36). 10/2012; 3(10). DOI: 10.18632/oncotarget.714
Source: PubMed


Dysregulation of the pathways that preserve mitochondrial integrity hallmarks many human diseases including diabetes, neurodegeration, aging and cancer. The mitochondrial citrate transporter gene, SLC25A1 or CIC, maps on chromosome 22q11.21, a region amplified in some tumors and deleted in developmental disorders known as velo-cardio-facial- and DiGeorge syndromes. We report here that in tumor cells CIC maintains mitochondrial integrity and bioenergetics, protects from mitochondrial damage and circumvents mitochondrial depletion via autophagy, hence promoting proliferation. CIC levels are increased in human cancers and its inhibition has anti-tumor activity, albeit with no toxicity on adult normal tissues. The knock-down of the CIC gene in zebrafish leads to mitochondria depletion and to proliferation defects that recapitulate features of human velo-cardio-facial syndrome, a phenotype rescued by blocking autophagy. Our findings reveal that CIC maintains mitochondrial homeostasis in metabolically active, high proliferating tissues and imply that this protein is a therapeutic target in cancer and likely, in other human diseases.

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Available from: Anton Wellstein, Jan 07, 2015
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    • "Based on the results of our previous investigations, a daily nutritional supplementation was designed to include selenomethionine (200 μg/d, Pure Encapsulations © , pro medico Handels GmbH, Graz, Austria) and a magistral formulation of magnesium citrate powder at a dose of 3 × 1.4 mmol/day. The choice of magnesium citrate is based on the beneficial effects in energetics as shown by Siekmeyer et al. [36] as well as on the positive effect on mitochondrial homeostasis [37]. "
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    ABSTRACT: The aim of this study was to discern whether a relation between biochemical parameters, sonography and musculoskeletal data exists in cases of hyperthyroidism and whether they are modifiable through supplementation with selenomethionine and magnesium citrate as well as by acupuncture and manual medicine methods.ResultsA direct correlation between whole blood selenium and serum magnesium was found in subjects without thyroid disease and in menopausal women while it was reversed in cases of thyroid diseases as well as in patients with depression, infection, and in infertile women. Vascularization indices were elevated in cases of newly diagnosed benign thyroid diseases. Musculoskeletal changes i.e. lateral tension and idiopathic moving toes, as well as situations of physical and psychological stress and minor trauma and infection led to an increase of vascularization. Magnesium levels correlated negatively with these two conditions. The supplementation brought a reduction of the vascularization indices and reduced the incidence of idiopathic moving toes. Treatment of lateral tension required manual medicine methods and acupuncture (gastrocnemius). A small subgroup of patients showed a further reduction of hyper-vascularization after receiving coenzyme Q10.Conclusions We interpret the elevated thyroid vascularization and low magnesium levels as signs of an inflammatory process related to the musculoskeletal changes. Improvement of thyroid function and morphology can be achieved after correcting the influence of stressors together with the supplementation regime. We hypothesize that the central biochemical event in thyroid disease is that of an acquired, altered mitochondrial function due to deficiency of magnesium, selenium, and coenzyme Q10.
    Full-text · Article · Nov 2014 · Biochimica et Biophysica Acta - Clinical
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    • "e l s e v i e r . c o m / l o c a t e / b b a g r m or in children harboring germ line SLC25A1 mutations that inactivate the citrate export pathway, leads to severe mitochondrial dysfunction [8] [9] [10]. Therefore, CIC plays a key role in both the mitochondrial and cytoplasmic pathways of energy production. "
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    ABSTRACT: The chronic induction of inflammation underlies multiple pathological conditions, including metabolic, autoimmune disorders and cancer. The mitochondrial citrate carrier (CIC), encoded by the SLC25A1 gene, promotes the export of citrate from the mitochondria to the cytoplasm, a process that profoundly influences energy balance in the cells. We have previously shown that SLC25A1 is a target gene for lipopolysaccharide signaling and promotes the production of inflammatory mediators. We now demonstrate that SLC25A1 is induced at the transcriptional level by two key pro-inflammatory cytokines, tumor necrosis factor-α (TNFα) and interferon-γ (IFNγ), and such induction involves the activity of the nuclear factor kappa B and STAT1 transcription factors. By studying the down-stream events following SLC25A1 activation during signals that mimic inflammation, we demonstrate that CIC is required for regulating the levels of nitric oxide and of prostaglandins by TNFα or IFNγ. Importantly, we show that the citrate exported from mitochondria via CIC and its downstream metabolic intermediate, acetyl-coenzyme A, are necessary for TNFα or IFNγ to induce nitric oxide and prostaglandin production. These findings provide the first line of evidence that the citrate export pathway, via CIC, is central for cytokine-induced inflammatory signals and shed new light on the relationship between energy metabolism and inflammation.
    Full-text · Article · Nov 2014 · Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms
    • "Several regulatory transcription factors, such as Sp1 (Iacobazzi et al., 2008) or NF-κB, can induce or inhibit CIC expression, thus linking CIC and cytosolic citrate levels with pro-inflammatory pathways (Infantino et al., 2011). In tumor cells, CIC was found to be transcriptionally upregulated and its inhibition has anti-tumor activity, potentially opening a therapeutic window for specific CIC inhibitors such as 1,2,3-benzene- tricarboxylate (BTA) or siRNA (Catalina-Rodriguez et al., 2012). Furthermore , citrate-mediated changes of fructose-2,6-bisphosphate (F2, 6P) levels significantly influence tumor cell proliferation. "
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    ABSTRACT: The majority of cancers demonstrate various tumor-specific metabolic aberrations, such as increased glycolysis even under aerobic conditions (Warburg effect), whereas mitochondrial metabolic activity and their contribution to cellular energy production are restrained. One of the most important mechanisms for this metabolic switch is the alteration in the abundance, utilization, and localization of various mitochondrial substrates. Numerous lines of evidence connect disturbances in mitochondrial metabolic pathways with tumorigenesis and provide an intriguing rationale for utilizing mitochondria as targets for anticancer therapy.
    No preview · Article · Aug 2014 · Mitochondrion
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