Involvement of Glyceraldehyde-3-phosphate Dehydrogenase in the X-Ray Resistance of HeLa Cells

Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University, Chiba, Japan.
Bioscience Biotechnology and Biochemistry (Impact Factor: 1.06). 10/2008; 72(9):2432-5. DOI: 10.1271/bbb.80168
Source: PubMed


We investigated changes in the sub-cellular distribution of glycelaldehyde-3-phosphate dehydrogenase (GAPDH) after X-ray irradiation in HeLa cells. Twenty-four h after irradiation at 5 Gy, nuclear GAPDH levels increased 2.6-fold, whereas total GAPDH levels increased only 1.2-fold. Knockdown of GAPDH using specific small interfering RNA (siRNA) led to sensitization to X-ray-induced cell death. These results suggest that GAPDH plays a role in the radioresponse.

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