Various Spatiotemporal Expression Profiles of Anther-Expressed Genes in Rice

Faculty of Science, Tohoku University, Sendai, 980-8587 Japan
Plant and Cell Physiology (Impact Factor: 4.93). 10/2008; 49(10):1417-28. DOI: 10.1093/pcp/pcn128
Source: PubMed


The male gametophyte and tapetum play different roles during anther development although they are differentiated from the same cell lineage, the L2 layer. Until now, it has not been possible to delineate their transcriptomes due to technical difficulties in separating the two cell types. In the present study, we characterized the separated transcriptomes of the rice microspore/pollen and tapetum using laser microdissection (LM)-mediated microarray. Spatiotemporal expression patterns of 28,141 anther-expressed genes were classified into 20 clusters, which contained 3,468 (12.3%) anther-enriched genes. In some clusters, synchronous gene expression in the microspore and tapetum at the same developmental stage was observed as a novel characteristic of the anther transcriptome. Noteworthy expression patterns are discussed in connection with gene ontology (GO) categories and gene annotations, which are related to important biological events in anther development, such as pollen maturation, pollen germination, pollen tube elongation and pollen wall formation.

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Available from: Nobuhiro Tsutsumi
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    • "Anther spatiotemporal expression profile in rice showed that 28,141 anther-expressed genes were classified into 20 clusters, which contained 3468 antherenriched genes. These genes are related to important biological events in anther development, such as pollen maturation, pollen germination, pollen tube elongation and pollen wall formation [25]. Mutations of these genes may cause male gamete sterility. "
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    • "EBOXBNNAPA (CANNTG) is E-box sequence responsible for light responsiveness and is regulated by bHLH and MYB-transcription factor in directing tissue-specific expression [13]. GATABOX (GATA) is the binding site for transcription factors with a zinc finger motif [85], GT1CONSENSUS (GRWAAW) recognizes GT-1 proteins which have tri-helix DNA-binding domains [86] and GTGANTG10 (GTGA) is a pollen-specific cis-elements [87]. MYCCONSENSUSAT (CANNTG) is a MYC recognition site which regulates transcription of genes under cold conditions by a MYC-like bHLH transcriptional activator [88], POLLEN1LELAT52 (AGAAA) is a regulatory element responsible for pollen-specific activation of gene expression [89], WBOXNTERF3 (TGACY) is a W-box promoter motif functioning in response to wound signal [90] and WRKY71OS (TGAC) is a binding site of rice WRKY71, a transcriptional repressor of gibberellin signaling pathway [91]. "
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    • "Multiple transcriptomes and several proteomes have been reported for whole anthers, utilizing organs that have completed cell specification and are proceeding through meiosis (Lu et al. 2006; Ma et al. 2007; Wijeratne et al. 2007; Skibbe et al. 2009; Wang et al. 2010a; Aya et al. 2011; Deveshwar et al. 2011; Nan et al. 2011) and for anther developmental stages after meiosis (Ma et al. 2008). Using manual or laser capture microdissection, transcriptomes are also available for isolated meiotic plant cells (Tang et al. 2010; Schmidt et al. 2011; Yang et al. 2011) and for isolated sporophytic anther tissues such as the tapetum (Hobo et al. 2008; Huang et al. 2009). Mature pollen, containing the vegetative cell and sperm, has also been characterized from multiple species (Honys and Twell 2004; Ma et al. 2006, 2008; Calarco et al. 2012). "
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