In vitro activity of daptomycin against Gram-positive cocci: the first multicentre study in Greece

Article (PDF Available)inInternational Journal of Antimicrobial Agents 32(6):525-8 · September 2008with20 Reads
DOI: 10.1016/j.ijantimicag.2008.05.020 · Source: PubMed
A total of 10420 Gram-positive cocci (including staphylococci, enterococci and various groups of streptococci) collected from clinically significant specimens in ten Greek hospitals during 2006--2007 were tested for their susceptibility to daptomycin. The minimum inhibitory concentration (MIC) was determined by the broth microdilution method. Daptomycin demonstrated very high activity against Enterococcus faecalis (MIC at which 50% of the isolates were inhibited (MIC50) = 1mg/L and MIC at which 90% of the isolates were inhibited (MIC90) = 1.36 mg/L), Enterococcus faecium (MIC50 = 1.36 mg/L and MIC90 = 1.90 mg/L), Streptococcus pyogenes (MIC50 = 0.12 mg/L and MIC90 = 0.50mg/L), Streptococcus agalactiae (MIC50 = 0.09 mg/L and MIC90 = 0.12 mg/L), Streptococcus pneumoniae (MIC50 = 0.24 mg/L and MIC90 = 0.5 mg/L) and viridans group streptococci (MIC50 = 0.50 mg/L and MIC90 = 0.89 mg/L). Resistance to linezolid and vancomycin for enterococci and to penicillin for streptococci appears to be independent of reduced susceptibility to daptomycin. On the other hand, daptomycin was also active against meticillin-resistant Staphylococcus aureus (MIC50 = 0.44 mg/L and MIC90 = 0.78 mg/L) and meticillin-resistant coagulase-negative staphylococci (MIC50 = 0.24 mg/L and MIC90 = 0.44 mg/L); however, 0.9% of the staphylococci tested had an MIC > 1mg/L, which is the Clinical and Laboratory Standards Institute breakpoint proposed for susceptibility. For all tested organism groups, resistance to daptomycin was not associated with glycopeptide resistance.
    • "The programme also found quinupristin/dalfopristin to be inactive against 10% of E. faecium isolates.7 As expected, daptomycin activity against enterococci was not influenced by their susceptibility to ampicillin or quinupristin/dalfopristin.22,45 Furthermore, of 1000 E. faecium clinical isolates tested in Greece, 2.5% were resistant to linezolid and 15% were resistant to quinupristin/dalfopristin, but none was resistant to daptomycin. As in previous studies, there were no differences in daptomycin MICs for isolates that were resistant or susceptible to other antibiotics. "
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