Indirect immunofluorescence as a diagnostic tool for parvovirus infection of broiler chickens

ArticleinAvian Pathology 14(2):269-73 · May 1985with7 Reads
DOI: 10.1080/03079458508436229 · Source: PubMed
Nuclear fluorescence was seen in the epithelial cells of the duodenum of broiler chicks infected experimentally at 1-day-old with a parvovirus of chicken origin. No antigenic relationship was detected by this method between chicken and goose parvoviruses.
    • "Positive nuclear staining was obtained by indirect immunoperoxidase staining in the epithelial cells of the small intestine of chickens experimentally infected with the designated ABU strain (Kisary et al. 1984; Kisary, 2001). Immunofluorescence staining was suggested as a diagnostic tool for parvoviral infection in chicken broilers (Kisary, 1985b). A recent survey revealed the presence of parvovirus in chicken and turkey samples from 8 different states in the USA. "
    [Show abstract] [Hide abstract] ABSTRACT: The major enteric disease (ED) complex in broiler chickens is runting-stunting syndrome and in turkey broilers is poult enteritis mortality syndrome. Viruses from numerous families have been identified in the intestinal tracts of poultry with ED, such as Astroviridae, Coronaviridae, Reoviridae, Rotaviridae, and Parvoviridae. The objective of the present study was to directly demonstrate the presence of the scarcely known chicken parvovirus (ChPV) and turkey parvovirus (TuPV) in Hungarian flocks experiencing clinical signs of ED. ChPV and TuPV infection were demonstrated in 15 chicken flocks and two turkey flocks, in intestinal samples collected between 2008 and 2010. The histopathological investigation revealed enteritis in the duodenum and jejunum, and atrophy of the lymphoid organs. Indirect immunohistochemistry (IHC) suggested the intestinal epithelium of chickens and turkeys as a potential replication site of the virus, similarly to other parvoviruses, while in case of the turkey samples IHC positivity was also observed in the bursa of Fabricius, liver and pancreas. However, no direct connection could be established between the presence of the pathogen in the above-mentioned tissues and the histopathological changes observed in the investigated flocks. The phylogenetic analysis performed on the partial nucleic acid sequence of the NS1 gene revealed an evident clustering tendency of the ChPV and TuPV strains, but also highlighted the potential reciprocal role of these two species in the epidemiology of these viruses. The role of the ChPV and TuPV in the ED is far from understood, but the results of the present study emphasize the fact that in certain, still not fully elucidated conditions, ChPV and TuPV may participate in the emergence of ED in chicken flocks, as suggested by previous experimental infections.
    Full-text · Article · Apr 2011
    • "EM examination of intestinal contents should be done after CsCl-gradient ultracentrifugation to eliminate most other small round viruses, and this method should be accompanied by biochemical studies aimed at characterizing the viral genome (Kisary et al., 1984). A simple, rapid FA procedure has been described, but this procedure requires specific antiserum that is not widely available (Kisary, 1985b). A parvovirus-like virus of turkeys was described by Trambel et al. (1982). "
    [Show abstract] [Hide abstract] ABSTRACT: Several different viruses have been identified as causes of gastrointestinal tract infections in poultry. These include rotaviruses, coronaviruses, enteroviruses, adenoviruses, astroviruses, and reoviruses. In addition, a number of other viruses of unknown importance have been associated with gastrointestinal diseases in poultry based on electron microscopic examination of feces and intestinal contents. Viral infections of the gastrointestinal tract of poultry are known to negatively impact poultry production, and they likely contribute to the development of other, extragastrointestinal diseases. Our current understanding of the viruses that cause gastrointestinal tract infections in poultry is reviewed, with emphasis given to those of greatest importance.
    Full-text · Article · Sep 1998
  • [Show abstract] [Hide abstract] ABSTRACT: The nucleic acid of chicken parvovirus-like particles showed sensitivity to DNase and S1 nuclease treatment and resistance to digestion with RNase. Viral DNA readily served as a template for self-primed conversion in vitro into a double-stranded form of about 5200 base pairs. There was no evidence for encapsidation of strands of opposite polarities. These findings confirm the taxonomic classification of chicken parvovirus-like particles as fowl parvovirus type 1 within the Parvovirus genus of the Parvoviridae.
    Full-text · Article · Nov 1985
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