Fluorescence Imaging of Intracellular Cadmium Using a Dual-Excitation Ratiometric Chemosensor
We described here a coumarin-based dual-excitation ratiometric probe for cadmium, CadMQ. This fluorescence sensor has high quantum yields of 0.59 and 0.70 in the metal-free and Cd(2+) bound forms, respectively, and has a dissociation constant of 0.16 nM for Cd(2+). CadMQ is cell permeable and locates within the acidic compartments of the cells. We further show that CadMQ is a useful tool to ratiometrically probe the change in the intracellular Cd(2+) levels with the use of two excited wavelengths.
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