Prevalence of Bartonella species antibodies and Bartonella species DNA in the blood of cats with and without fever

ArticleinJournal of Feline Medicine & Surgery 11(2):141-8 · September 2008with11 Reads
DOI: 10.1016/j.jfms.2008.06.005 · Source: PubMed
Abstract
The purpose of this study was to determine whether there are associations between Bartonella species antibody (enzyme-linked immunosorbent assay (ELISA) and Western blot (WB)) and polymerase chain reaction assay results in cats with and without fever. Afebrile control cats (39/93; 42.0%) were more likely to have Bartonella species antibodies than cats with fever (29/93; 31.2%). The difference in prevalence of Bartonella species deoxyribonucleic acid (DNA) in blood of cats with fever (14/81; 17.3%) as compared to afebrile control cats (6/81; 7.4%) approached statistical significance (P=0.0571). Bartonella species ELISA or WB results frequently did not correlate to the presence or absence of Bartonella species DNA in blood. The results of this study indicate that in cats, Bartonella species antibody tests cannot predict whether fever is due to Bartonella species infection and should not be used to determine the Bartonella species infection status.
    • "In this study, 28 cats with serum IgG to B. henselae were negative for B. henselae DNA in blood while 6 cats with B.henselae DNA in blood were seronegative. These results have supported the results of Lappin and Hawley [19] and Nasoiu et al. [2] . It is clear that the clinicians should focus on cats which are reservoir of the CSD and on preventing this zoonotic disease. "
    Full-text · Article · Jan 2016
    • "enzyme-linked immunosorbent assay (ELISA) that uses B. henselae as the antigen source. This assay detects antibodies against both B. henselae and B. clarridgeaie; the lowest positive titer is defined as 1:64 (Lappin et al., 2009). FELV and FIV testing: Serum samples were assayed at CSU for FeLVantigen and FIV antibodies using commercial kits (SNAPH FeLV/ FIV, IDEXX Laboratories, Portland, Maine). "
    File · Data · Oct 2015 · Virology
    • "Australia: Whole blood DNA from client-owned, domestic cats from Eastern Australia was available for FcaGHV qPCR (n ¼110) (Barrs et al., 2010). Data available for analyses were: sex, age (6 months—2 years or 42 years), neuter status, environment (indoor only or outdoor access), state of domicile (New South Wales, Queensland or Victoria), serology for Bartonella spp IgG (Lappin et al., 2009) and results of PCR assays for Mycoplasma haemofelis (Mhf), Candidatus Mycoplasma haemominutum (Mhm) (Jensen et al., 2001), Bartonella henselae and Bartonella clarridgeiae (Jensen et al., 2000). Cats were classified as either healthy or sick by the attending veterinarian based on the findings of a physical examination and, where available, medical history. "
    [Show abstract] [Hide abstract] ABSTRACT: Felis catus gammaherpesvirus 1 (FcaGHV1), recently discovered in the USA, was detected in domestic cats in Australia (11.4%, 95% confidence interval 5.9–19.1, n=110) and Singapore (9.6%, 95% confidence interval 5.9–14.6, n=176) using qPCR. FcaGHV1 qPCR positive cats were 2.8 times more likely to be sick than healthy. Risk factors for FcaGHV1 detection included being male, increasing age and coinfection with pathogenic retroviruses, feline immunodeficiency virus (FIV) or feline leukaemia virus. FcaGHV1 DNA was detected in multiple tissues from infected cats with consistently high virus loads in the small intestine. FcaGHV1 viral load was significantly higher in FIV-infected cats compared with matched controls, mimicking increased Epstein–Barr virus loads in human immunodeficiency virus-infected humans. FcaGHV1 is endemic in distant geographic regions and is associated with being sick and with coinfections. Horizontal transmission of FcaGHV1 is supported, with biting being a plausible route. A pathogenic role for FcaGHV1 in domestic cats is supported.
    Full-text · Article · Jul 2014
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