Article

A GAL4-Driver Line Resource for Drosophila Neurobiology

Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA.
Cell Reports (Impact Factor: 8.36). 10/2012; 2(4). DOI: 10.1016/j.celrep.2012.09.011
Source: PubMed

ABSTRACT

We established a collection of 7,000 transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer. We used confocal microscopy of dissected nervous systems to determine the expression patterns driven by each fragment in the adult brain and ventral nerve cord. We present image data on 6,650 lines. Using both manual and machine-assisted annotation, we describe the expression patterns in the most useful lines. We illustrate the utility of these data for identifying novel neuronal cell types, revealing brain asymmetry, and describing the nature and extent of neuronal shape stereotypy. The GAL4 lines allow expression of exogenous genes in distinct, small subsets of the adult nervous system. The set of DNA fragments, each driving a documented expression pattern, will facilitate the generation of additional constructs for manipulating neuronal function.

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    • "The presynaptic terminals of PAM-α1 are localized to the basal compartment of the MB α lobe (α1) (Liu et al., 2012), whereas the dendrites stretch horizontally across the superior medial, intermediate, and lateral protocerebra (SMP, SIP, and SLP, respectively) (Aso et al., 2014a; Yamagata et al., 2015) (Figure 1A,B,H). By segmenting the dendrites of PAM-α1 in confocal images (Aso et al., 2014a), we queried a database of GAL4 expression patterns (Jenett et al., 2012) to computationally search for candidate upstream neurons. Strikingly, one of the input candidates we identified was the output neurons from the α1 compartment of the MB (MBON-α1) (Figure 1C,D). "
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    • "The selective activation of single classes of sensory cells allows us to disambiguate sensory cues and determine the contribution of specific sensory inputs. PPN1 neurons respond to F cell stimulation and activate P1 neurons As F cells terminating in the VNC do not directly contact P1, we screened existing Gal4 collections (Gohl et al., 2011; Jenett et al., 2012) for neurons that might contact F cell projections and project to higher brain regions. We identified a pair of neurons with dendrites in the VNC and axons in the protocerebrum, marked specifically by R56C09-Gal4, which we name Pheromone Projection Neuron Figure 1. "
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    • "An intersectional screen identifies fru M + second-and third-order auditory neurons Motivated by the hypothesis that fru M labels neurons that detect courtship-relevant sensory stimuli (Manoli et al., 2005; Stockinger et al., 2005), we performed an anatomical screen aimed at identifying fru M + neurons in the auditory pathway. Specifically, ∼1000 cis regulatory module (CRM) GAL4 lines with relatively sparse neuronal expression patterns (Jenett et al., 2012) were crossed to LexAop2-FLP; fru LexA , UAS>stop>myr::GFP to restrict expression of GFP to those neurons that express both GAL4 and fru LexA (Figure 1J). These intersectional expression patterns were then registered onto a standard brain for analysis of potentially overlapping projection patterns. "
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