Structure-function analysis indicates that sumoylation modulates DNA-binding activity of STAT1

BMC Biochemistry (Impact Factor: 1.44). 10/2012; 13(1):20. DOI: 10.1186/1471-2091-13-20
Source: PubMed


STAT1 is an essential transcription factor for interferon-γ-mediated gene responses. A distinct sumoylation consensus site (ψKxE) 702IKTE705 is localized in the C-terminal region of STAT1, where Lys703 is a target for PIAS-induced SUMO modification. Several studies indicate that sumoylation has an inhibitory role on STAT1-mediated gene expression but the molecular mechanisms are not fully understood.

Here, we have performed a structural and functional analysis of sumoylation in STAT1. We show that deconjugation of SUMO by SENP1 enhances the transcriptional activity of STAT1, confirming a negative regulatory effect of sumoylation on STAT1 activity. Inspection of molecular model indicated that consensus site is well exposed to SUMO-conjugation in STAT1 homodimer and that the conjugated SUMO moiety is directed towards DNA, thus able to form a sterical hindrance affecting promoter binding of dimeric STAT1. In addition, oligoprecipitation experiments indicated that sumoylation deficient STAT1 E705Q mutant has higher DNA-binding activity on STAT1 responsive gene promoters than wild-type STAT1. Furthermore, sumoylation deficient STAT1 E705Q mutant displayed enhanced histone H4 acetylation on interferon-γ-responsive promoter compared to wild-type STAT1.

Our results suggest that sumoylation participates in regulation of STAT1 responses by modulating DNA-binding properties of STAT1.

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Available from: Olli Silvennoinen, Apr 09, 2014
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    • "SUMOylation is a post-transcriptional modification that, in the case of STAT1, seems to function as a negative regulator, since inhibits STAT1 Tyr701 phosphorylation, prevents STAT1 DNA binding and also promotes its dephosphorylation [40], [51]–[53]. Thus, p38 MAPK induced maximization of STAT1 transcriptional potential (by phosphorylation on Ser727) and this event would precede the relatively slow emergence of STAT1 SUMOylation, triggering a negative feedback loop through PIAS1 and SUMO recruitment [52]. Remarkably, our data show that cytokine-induced activation (phosphorylation) of p38 MAPK was not counteracted by Resv and/or 5-ASA. "
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