Proportions of Mycobacterium massiliense and Mycobacterium bolletii Strains among Korean Mycobacterium chelonae-Mycobacterium abscessus Group Isolates

Department of Microbiology, Cancer Research Institute, SNUMRC, Seoul National University College of Medicine, and Seoul National University Hospital, Seoul 110-799, Republic of Korea.
Journal of clinical microbiology (Impact Factor: 3.99). 09/2008; 46(10):3384-90. DOI: 10.1128/JCM.00319-08
Source: PubMed


Korean isolates of the Mycobacterium chelonae-Mycobacterium abscessus group, which had been isolated from two different hospitals in South Korea, were identified by PCR restriction analysis (PRA)
and comparative sequence analysis of 16S rRNA genes, rpoB, and hsp65 to evaluate the proportion of four closely related species (M. chelonae, M. abscessus, M. massiliense, and M. bolletii). Of the 144 rapidly growing mycobacterial strains tested, 127 strains (88.2%) belonged to the M. chelonae-M. abscessus group. In this group, M. chelonae, M. abscessus, M. massiliense, and M. bolletii accounted for 0.8% (n = 1), 51.2% (n = 65), 46.5% (n = 59), and 1.6% (n = 2), respectively. Two isolates which showed discordant results, M. massiliense by rpoB sequence analysis and M. abscessus by hsp65 sequence analysis, were finally identified as M. massiliense based on the additional analysis of sodA and the 16S-23S internal transcribed spacer. M. abscessus group I isolates previously identified by hsp65 PRA were all found to be M. abscessus, whereas group II isolates were further identified as M. massiliense or M. bolletii by sequencing of rpoB and hsp65. Smooth, rough, or mixed colonies of both M. abscessus and M. massiliense isolates were observed. M. massiliense strains that were highly resistant to clarithromycin had a point mutation at the adenine at position 2058 (A2058) or 2059 (A2059) in the peptidyltransferase region of the 23S rRNA gene.

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Available from: Bum-Joon Kim
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    • "M. abscessus complex (MABC) is the second most common etiologic organism (14-33%) of NTM lung disease in Korea17,18,19,24. Within MABC, M. abscessus (44-53%) and M. massiliense (45-55%) are equally distributed, while M. bolletii is a relatively rare pathogen (1-2%) in Korea25,26,27. In addition, M. kansasii is infrequently isolated and a relatively uncommon cause of NTM lung disease in Korea28,29. "
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    • "However, interspecific relationships have been identified within the M. abscessus group through genotype analysis, such as PCR restriction analysis (PRA) and sequencing of hsp65 and rpoB. Moreover, it has been recently reported that M. massiliense and M. bolletii are very closely related to M. abscessus [10-12, 19, 20]. In the present study M. abscessus and M. massiliense strains were differentiated on the basis of the erm(41) gene, and M. abscessus and M. bolletii were differentiated utilizing the -35 sequence difference in erm(41) gene promoter. "
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    • "In particular, a recent study based on whole genome sequencing revealed the first evidence of human to human transmission in NTM infection, by proving its transmission between cystic fibrosis patients; suggesting unusually high infectivity of humans by M. massiliense strains [36]. In South Korea, a distinct epidemiologic trend was reported [37], of higher prevalence of M. massiliense strains (of the M. abscessus − complex); part of which may be attributed to the presence of the M. massiliense Type II genotype found only in Korean patients [20]. "
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