BACKGROUND: While increasing evidence suggests the negative effects of physical inactivity on individual job productivity1 and the negative associations between high occupational sitting times and employee’s health2, the relationship between time spent sitting at work and job performance remains unclear. PURPOSE: Prior to the beginning of a pedometer-based program to reduce occupational sitting, this study examined associations between time spent sitting at work, job productivity and related work loss in white-collar, university employees. METHODS: Five hundred and fifty-seven participants from four Spanish universities (42±22 years old, BMI 24,07±3.7 kg/mt2, 62% women, 73% working full time, 58% academic and 42% administrative staff) completed a survey measuring time spent sitting at work3 (Domain-specific sitting questionnaire), work performance (Work Limitations Questionnaire, WLQ) 4 and an estimation of work productivity loss based on WLQ data (WLQ Index score)4. Work performance was identified using three subscales, reflecting ability to meet that day’s demands for (i) output (ii) time management and (iii) mental and interpersonal skills. Differences in these subscales and the WLQ index score were compared across sitting time tertiles using ANOVA. RESULTS: Employees that spent less time sitting while working (113±50 min/day, n=184) showed significantly better skills in performing job’s time and scheduling demands (F= 6.9, p=0,001) compared to employees that sat more (309±47 min/day, n=201; 462±69 min/day, n=172). Scores in the WLQ Index estimated that those sitting more (462±69 min/day) showed a 5.8% decrease in productivity compared to the 3.9% shown by those who sat less (113±50 min/day) although the difference was not significant (p=0.061). CONCLUSION: Preliminary data indicate that levels of sitting at work were associated with the ability to perform specific job demands in our sample. On going work will assess the impact sitting interventions have on employee work performance and job productivity loss.