Early determination of sex in Jojoba plant by CAPS assay

The Journal of Agricultural Science (Impact Factor: 0.65). 05/2011; 149(03):327 - 336. DOI: 10.1017/S0021859610000948


Jojoba [Simmondsia chinensis (Link) Schneider] is a dioecious plant grown for its seeds, which are the source of liquid wax or jojoba oil. The sex of jojoba plants cannot be determined with morphological characters until the plants reach reproductive maturity at 3 or more years old. This difficulty of early sex determination imposes severe constraints in breeding studies and in the sex allocation of seedlings in seed orchard establishment, and importantly in a priori mating designs to produce superior jojoba individuals. This study reports three new cleavage-amplified polymorphic sequence (CAPS) assays, which identify male and female individuals distinctly. One of the assays could also identify hermaphrodite jojoba plants existing in nature or obtained using mutagenesis studies.

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Available from: Mehmet Karaca, Dec 13, 2014
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    • "In the past few years, several publications have appeared pertaining to sex linked marker in Jojoba (Agarwal et al., 2011; Agrawal et al., 2007; Heikrujam et al., 2014a, 2014b; Hosseini et al., 2011; Ince et al., 2010, 2011 and Sharma et al., 2008) however, there are scant information regarding molecular diversity among different genotypes (Bhardwaj et al., 2010; Sharma et al., 2009). "
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    ABSTRACT: To detect genetic variations among different Simmondsia chinensis genotypes, two gene targeted markers, start codon targeted (SCoT) polymorphism and CAAT box-derived polymorphism (CBDP) were employed in terms of their informativeness and efficiency in analyzing genetic relationships among different genotypes. A total of 15 SCoT and 17 CBDP primers detected genetic polymorphism among 39 Jojoba genotypes (22 females and 17 males). Comparatively, CBDP markers proved to be more effective than SCoT markers in terms of percentage polymorphism as the former detecting an average of 53.4% and the latter as 49.4%. The Polymorphic information content (PIC) value and marker index (MI) of CBPD were 0.43 and 1.10, respectively which were higher than those of SCoT where the respective values of PIC and MI were 0.38 and 1.09. While comparing male and female genotype populations, the former showed higher variation in respect of polymorphic percentage and PIC, MI and Rp values over female populations. Nei's diversity (h) and Shannon index (I) were calculated for each genotype and found that the genotype “MS F” (in both markers) was highly diverse and genotypes “Q104 F” (SCoT) and “82–18 F” (CBDP) were least diverse among the female genotype populations. Among male genotypes, “32 M” (CBDP) and “MS M” (SCoT) revealed highest h and I values while “58-5 M” (both markers) was the least diverse. Jaccard's similarity co-efficient of SCoT markers ranged from 0.733 to 0.922 in female genotypes and 0.941 to 0.746 in male genotype population. Likewise, CBDP data analysis also revealed similarity ranging from 0.751 to 0.958 within female genotypes and 0.754 to 0.976 within male genotype populations thereby, indicating genetically diverse Jojoba population. Employing the NTSYS (Numerical taxonomy and multivariate analysis system) Version 2.1 software, both the markers generated dendrograms which revealed that all the Jojoba genotypes were clustered into two major groups, one group consisting of all female genotypes and another group comprising of all male genotypes. During the present investigation, CBDP markers proved more informative in studying genetic diversity among Jojoba. Such genetically diverse genotypes would thus be of great significance for breeding, management and conservation of elite (high yielding) Jojoba germplasm.
    Full-text · Article · Sep 2015 · Meta Gene
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    • "All reagents used in DNA extraction and subsequent analyses, including polymerase chain reactions, were molecular biology grade purchased from Amresco (Solon, OH, USA) and Bioron. Concentration, amount, quality, and purity of the extracted DNA samples were determined using a spectrophotometer and agarose gel electrophoresis technique (Ince and Karaca, 2011b). "
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    ABSTRACT: Dianthus L. is one of the highly valued plant species in the family Caryophyllaceae. More than 30,000 cultivars of commercial carnations have been recorded and there is a need for an effective and cheap method to reveal their genetic diversity and identify the cultivars. To the best of our knowledge this is the first report of implementation of the touchdown direct amplification of minisatellite region DNA polymerase chain reaction (Td-DAMD-PCR) technique in the genus Dianthus. A total of 12 core minisatellite primers empirically selected from 22 primers were used in fingerprinting and phylogenetic studies of some commercial carnation cultivars. Analysis revealed that commercial carnations have a wide genetic base and they were probably obtained using inter-crosses between and among different species of the genus Dianthus. A total of 17 DNA markers were variety specific and most of the remaining markers obtained in the present study were useful in fingerprinting of the commercial varieties studied. Commercial varieties were differentiated in spray and standard carnation groups in a principal coordinate analysis and a Bayesian 50% majority-rule consensus tree. Td-DAMD-PCR markers reported in this study could be very useful in species identification, determination of genetic relationships, and phylogenetic studies of species of Dianthus.
    Full-text · Article · Apr 2015 · TURKISH JOURNAL OF BIOLOGY
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    • "Amplicons were separated using 2% (Td-DAMD-PCR) and 3% (Td-SSR) high resolution agarose gel (Serva, Heidelberg, Germany) electrophoresis according the procedures described in Ince et al. (2011). Amplified products in gels were visualized on an ultraviolet trans-illuminator to confirm amplifications, molecular mass and any observed length polymorphisms. "
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    ABSTRACT: Some farmers still grow common bean (Phaseolus vulgaris L.) landraces for self-consumption in developing countries although many landraces are being replaced with modern cultivars. An effective and cheap method to reveal the genetic diversity in landraces is important. In this study, Td-DAMD-PCR, Td-SSR and CAPS-microsatellite techniques were compared using the same PCR amplification profile and reagents. Comparison analyses revealed that Td-DAMD-PCR markers amplified with 13 minisatellite primers, which were selected from 22 primers based on resolutions and reproducibility, were as reliable as Td-SSR and CAPS-microsatellite markers and produced more polymorphic markers, differentiating all the 24 common bean landraces. This study also showed that common bean landraces grown in Turkey contain great genetic variations. Td-DAMD-PCR markers amplified with selected 13 minisatellite primers can be effectively used in identification and preservation of common bean landraces that Td-SSR and CAPS-microsatellite markers could not reveal polymorphisms.
    Full-text · Article · Jan 2011 · Plant Omics
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