Hypolipidaemic and anti-atherosclerotic effects of lupin proteins in a rabbit model. Br J Nutr

The British journal of nutrition (Impact Factor: 3.45). 09/2008; 100(04):707 - 710. DOI: 10.1017/S000711450894215X


The biological activities of a protein isolate from lupin (Lupinus albus) were studied in a rabbit model of atherosclerosis. Focal plaque development was induced at both common carotid arteries by perivascular injury. After surgery, animals were fed three different diets for 90 d, all with 1 % cholesterol, 15 % SFA and 20 % protein; the protein source was casein (CAS), lupin proteins (LUP) or 50 % CAS+50 % LUP (CAS+LUP). Lower cholesterolaemia was detected in the LUP v. the CAS group at 60 and 90 d of treatment ( − 40·3 and − 33·5 %, respectively; P < 0·05). Cryosection analyses of the carotids indicated a significant reduction in focal lesion progression in the LUP v. the CAS group ( − 37·4 %; P < 0·05). In summary, in a rabbit model of atherosclerosis, a protein isolate from L. albus reduced cholesterolaemia and exerted a remarkable protective activity against atherosclerosis progression.

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Available from: Anna Arnoldi, Feb 07, 2014
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    • "soybean, beans, peanut and lupin) have been studied with the specific aim of identifying and characterizing the proteins accounting for their health-promoting properties [1,2] and/or allergenic effects [3,4]. Lupin seeds, which are increasingly used in Europe as an ingredient for bakery products or as a soy substitute [4], have been characterized in relation to their interesting anti-hypercholesterolemic [5,6,7,8] and anti-hyperglycemic effects [2,9,10]. "
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    ABSTRACT: The molecular characterization of bioactive food components is necessary for understanding the mechanisms of their beneficial or detrimental effects on human health. This study focused on γ-conglutin, a well-known lupin seed N-glycoprotein with health-promoting properties and controversial allergenic potential. Given the importance of N-glycosylation for the functional and structural characteristics of proteins, we studied the purified protein by a mass spectrometry-based glycoproteomic approach able to identify the structure, micro-heterogeneity and attachment site of the bound N-glycan(s), and to provide extensive coverage of the protein sequence. The peptide/N-glycopeptide mixtures generated by enzymatic digestion (with or without N-deglycosylation) were analyzed by high-resolution accurate mass liquid chromatography-multi-stage mass spectrometry. The four main micro-heterogeneous variants of the single N-glycan bound to γ-conglutin were identified as Man2(Xyl) (Fuc) GlcNAc2, Man3(Xyl) (Fuc) GlcNAc2, GlcNAcMan3(Xyl) (Fuc) GlcNAc2 and GlcNAc 2Man3(Xyl) (Fuc) GlcNAc2. These carry both core β1,2-xylose and core α1-3-fucose (well known Cross-Reactive Carbohydrate Determinants), but corresponding fucose-free variants were also identified as minor components. The N-glycan was proven to reside on Asn131, one of the two potential N-glycosylation sites. The extensive coverage of the γ-conglutin amino acid sequence suggested three alternative N-termini of the small subunit, that were later confirmed by direct-infusion Orbitrap mass spectrometry analysis of the intact subunit.
    Full-text · Article · Sep 2013 · PLoS ONE
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    • "The consumption of diets with an elevated content of saturated fats and cholesterol have a tendency to develop a non-alcoholic fatty liver disease, apart from other clinical states such as a resistance to insulin, diabetes type II and obesity (Ferré & Foufelle, 2010). Marchesi et al. (2008) studied the hypolipidemic and anti-atherogenic effect of lupin protein isolates (Lupinus albus) in rabbits and reported a significant reduction of cholesterol and a reduction of the risk of developing atherosclerosis. A point raised in this study was the capacity of intervention of this legume and its protein isolate in the case of hypercholesterolaemia and steatosis of the liver. "
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    ABSTRACT: a b s t r a c t This study describes the hypocholesterolaemic effect of whole lupin and its protein in hamsters. The diets were: casein (control group HC), lupin protein isolate (group HPI) and whole lupin seed (group HWS). Diets from HPI and HWS promoted a significant reduction of total cholesterol and non-HDL cholesterol in the hamsters' plasma as compared with HC. The true digestibility of HPI and HC groups were similar and differed significantly from the HWS one, which in turn showed a significant difference in total sterol excretion as compared to the former groups. Histological analysis of the liver revealed that animals fed on HPI and HWS diets presented a low level of steatosis (level 1) as compared to the ones fed on HC diet (level 4). Our findings demonstrate that protein isolate from Lupinus albus from Brazil has a metabolic effect on endogenous cholesterol metabolism and a protector effect on development of hepatic steatosis.
    Full-text · Article · Jan 2012 · Food Chemistry
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    • "In addition, there are increasing evidences of some potential health benefits, such as the hypocholesterolaemic (Martins et al., 2005; Sirtori et al., 2004), anti-atherogenic (Marchesi et al., 2008), hypotensive (Lee et al., 2009; Pilvi et al., 2006), and hypoglycaemic activities (Lee et al., 2006; Magni et al., 2004), demonstrated either by experiments on suitable animal models or by clinical trials. 0308-8146/$ -see front matter Ó 2011 Elsevier Ltd. "
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    ABSTRACT: A chemotyping and genotyping comprehensive approach may be useful for the analytical traceability of food ingredients. The interest for lupin (Lupinus spp.) is developing owing to the high protein percentage as well as the positive technological and nutraceutical properties. The objective was the development of innovative models for discerning between Lupinus albus and Lupinus angustifolius, the most used in human nutrition, by applying multivariate statistical analysis (Principal Component Analysis, PCA) and artificial intelligence (Self Organising Maps, SOMs) onto chemical parameters (proximate composition, alkaloids, tocopherols) or Random Polymorphic DNA fingerprints. The application of PCA to either chemical or genetic data permitted the effective discrimination between the two species, whereas the application of the SOM approach to both data-sets enabled clustering some cultivars. The possibility of discriminating L. albus from L. angustifolius is relevant for lupin traceability: the foreseen fields of application are refined flours or ingredients, where morphological analysis is not applicable.
    Full-text · Article · Dec 2011 · Food Chemistry
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