Article

Phytochemical composition and in vitro pharmacological activity of two rose hip (Rosa canina L.) preparations

Department of Pharmacognosy, Institute of Pharmaceutical Sciences, University of Graz, Universitaetsplatz 4, 8010 Graz, Austria.
Phytomedicine: international journal of phytotherapy and phytopharmacology (Impact Factor: 3.13). 09/2008; 15(10):826-35. DOI: 10.1016/j.phymed.2008.06.012
Source: PubMed

ABSTRACT

The aim of the present study was to compare powdered rose hip with and without fruits (Rosae pseudofructus cum/sine fructibus, Rosa canina L., Rosaceae) with regard to their phytochemical profile and their in vitro anti-inflammatory and radical-scavenging properties. The two powders were subsequently extracted with solvents of increasing polarity and tested for inhibition of cyclooxygenase (COX-1, COX-2) and of 5-LOX-mediated leukotriene B(4) (LTB(4)) formation as well as for DPPH-radical-scavenging capacity. While the water and methanol extracts were inactive in the COX-1, COX-2 and LTB(4) inhibition assays, the n-hexane and the dichloromethane extracts inhibited all three enzymes. In the active extracts, the triterpenoic acids ursolic acid, oleanolic acid and betulinic acid were identified, although only in minute amounts. Furthermore, oleic, linoleic and alpha-linolenic acid were identified apart from several saturated fatty acids. Even though unsaturated fatty acids are known to be good inhibitors of COX-1, COX-2 and LT formation, no clear correlation between their concentration in the extracts and their activity was found. We suggest that other, yet unidentified, lipophilic constituents might play a more important role for the observed in vitro inhibitory activity on arachidonic acid metabolism. Some of the extracts also showed considerable DPPH radical scavenging activity, the methanolic extracts being most potent. The radical scavenging activity of the extracts correlated very well with their total phenolic content, while ascorbic acid contributes only little to the radical-scavenging activity due to its low concentration present in the extracts. In summary, extracts derived from powdered rose hip without fruits were more effective in all assays carried out compared with extracts derived from powdered rose hip with fruits.

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    • "Rosa canina L. is a plant whose berries are endowed with antioxidant, anti-inflammatory, immunomodulating and Vet Res Commun antimicrobial activity due to the presence of phenolic acids, proanthocyanidins, tannins, flavonoids, unsaturated and polyunsaturated fatty acids, phospholipids, minerals, galactolipids, carotenoids and triterpenes (Chrubasik et al. 2008; Sadigh- Eteghad et al. 2011). This plant exerts a specific antiinflammatory activity (Jager et al. 2007, 2008; Larsen et al. 2003; Lattanzio et al. 2011; Wenzig et al. 2008), some immunomodulatory and antioxidant activities (Davitashvili et al. 2010; Sadigh-Eteghad et al. 2011; Sies et al. 1992; Takashima et al. 2012; Tumbas et al. 2012), and antimicrobial effects (Shiota et al. 2000). Additional activities ascribed to this plant are antiulcerogenic and probiotic (Deliorman Orhan et al. 2007; Gurbuz et al. 2003; Johansson et al. 1998), hypoglycemic (Ninomiya et al. 2007), antimutagenic and anticancerogenic (Trovato et al. 1996). "
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