A Fluorescent Alternative to the Synthetic Strigolactone GR24

Article (PDF Available)inMolecular Plant 6(1) · September 2012with247 Reads
DOI: 10.1093/mp/sss110 · Source: PubMed
Abstract
Strigolactones have recently been implicated in both above and below ground developmental pathways in higher plants. To facilitate the molecular and chemical properties of strigolactones in vitro and in vivo, we have developed a fluorescent strigolactone molecule, CISA-1, synthesised via a novel method which was robust, high-yielding and used simple starting materials. We demonstrate that CISA-1 has a broad range of known strigolactone activities and further report on an adventitious rooting assay in Arabidopsis which is a highly sensitive and rapid method for testing biological activity of strigolactone analogues. In this rooting assay and the widely used Orobanche germination assay CISA-1 showed stronger biological activity than the commonly tested GR24. CISA-1 and GR24 were equally effective at inhibiting branching in Arabidopsis inflorescence stems. In both the branching and adventitious rooting assay we also demonstrated that CISA-1 activity is dependent on the max strigolactone signalling pathway. In water methanol solutions, CISA-1 was about 3 fold more stable than GR24, which may contribute to the increased activity observed in the various biological tests.

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Molecular Plant Volume 6 Number 1 Pages 100–112 January 2013 RESEARCH ARTICLE
A Fluorescent Alternative to the Synthetic
Strigolactone GR24
AmandaRasmussen
a
, ThomasHeugebaert
b
, CedrickMatthys
c,d
, RikVan Deun
e
, Francois-DidierBoyer
f
,
SofieGoormachtig
c,d
, ChristianStevens
b
and DannyGeelen
a,1
a Plant Production, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, Ghent 9000, Belgium
b Research Group SynBioC, Department of Sustainable Organic Chemistry and Technology, Faculty of Bioscience Engineering, Ghent University, Coupure
links 653, B-9000 Gent, Belgium
c Department of Plant Systems Biology, VIB, Ghent 9052, Belgium
d Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent 9052, Belgium
e f-element Coordination Chemistry Lab, Inorganic and Physical Chemistry Department, Ghent University, Krijgslaan 281—S3, 9000 Gent, Belgium
f Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles, UPR2301 CNRS, INRA, 1 avenue de la Terrasse, F-91198 Gif-sur-Yvette Cedex, France
ABSTRACT Strigolactones have recently been implicated in both above- and below-ground developmental pathways
in higher plants. To facilitate the molecular and chemical properties of strigolactones in vitro and in vivo, we have devel-
oped a fluorescent strigolactone molecule, CISA-1, synthesized via a novel method which was robust, high-yielding, and
used simple starting materials. We demonstrate that CISA-1 has a broad range of known strigolactone activities and
further report on an adventitious rooting assay in Arabidopsis which is a highly sensitive and rapid method for testing
biological activity of strigolactone analogs. In this rooting assay and the widely used Orobanche germination assay,
CISA-1 showed stronger biological activity than the commonly tested GR24. CISA-1 and GR24 were equally effective at
inhibiting branching in Arabidopsis inflorescence stems. In both the branching and adventitious rooting assay, we also
demonstrated that CISA-1 activity is dependent on the max strigolactone signaling pathway. In water methanol solu-
tions, CISA-1 was about threefold more stable than GR24, which may contribute to the increased activity observed in
the various biological tests.
Key words: strigolactones; adventitious rooting; branching; fluorescent markers; parasitic wee