CHD1L Protein is overexpressed in human ovarian carcinomas and is a novel predictive biomarker for patients survival

BMC Cancer (Impact Factor: 3.36). 09/2012; 12(1):437. DOI: 10.1186/1471-2407-12-437
Source: PubMed


Our recent studies suggested that the chromodomain helicase DNA binding protein 1-like (CHD1L) gene plays an oncogenic role in human hepatocellular carcinoma. However, the status of CHD1L protein expression in ovarian cancer and its clinical/prognostic significance are obscure.

In this study, immunohistochemistry (IHC) for CHD1L was performed on a tissue microarray (TMA) containing 102 primary ovarian carcinomas and 44 metastatic lesions (omental metastasis). Receiver-operator curve (ROC) analysis was used to evaluate patients’ survival status.

There is an augmented tendency of CHD1L expression in ovarian carcinoma metastasis than in primary lesions (P<0.05). A significant association was found between positive expression of CHD1L and tumors histological type (P <0.05). By univariate survival analysis of the ovarian carcinoma cohorts, positive expression of CHD1L was significantly correlated with shortened patient survival (mean 66.7 months versus 97.4 months, P<0.05). Moreover, CHD1L expression was evaluated to be a significant and independent prognostic factor in multivariate analysis (P<0.05).

These findings provide evidence that positive expression of CHD1L protein is significantly correlated with the metastasis proceeding of ovarian carcinoma, and CHD1L protein expression, as examined by IHC, may act as a novel prognostic biomarker for patients with ovarian carcinoma.

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    • "IHC was employed to measure the Ag-binding rates for TfRscFv-GAL4 fusion protein with various tumor TMA sections. The immunohistochemical study of Ag-binding was performed using a standard streptavidin-peroxidase method described previously [40]. The endogenous peroxidase activity was blocked with 3% H2O2 for 10 minutes. "
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