Ethnobotanical study of some of mosquito repellent plants in north-eastern Tanzania

Tropical Pesticides Research Institute, Division of Livestock and Human Disease Vectors Control, P,O, Box 3024, Arusha,Tanzania.
Malaria Journal (Impact Factor: 3.11). 02/2008; 7(1):152. DOI: 10.1186/1475-2875-7-152
Source: PubMed


The use of plant repellents against nuisance biting insects is common and its potential for malaria vector control requires evaluation in areas with different level of malaria endemicity. The essential oils of Ocimum suave and Ocimum kilimandscharicum were evaluated against malaria vectors in north-eastern Tanzania.
An ethnobotanical study was conducted at Moshi in Kilimanjaro region north-eastern Tanzania, through interviews, to investigate the range of species of plants used as insect repellents. Also, bioassays were used to evaluate the protective potential of selected plants extracts against mosquitoes.
The plant species mostly used as repellent at night are: fresh or smoke of the leaves of O. suave and O. kilimandscharicum (Lamiaceae), Azadirachta indica (Meliaceae), Eucalyptus globules (Myrtaceae) and Lantana camara (Verbenaceae). The most popular repellents were O. kilimandscharicum (OK) and O. suave (OS) used by 67% out of 120 households interviewed. Bioassay of essential oils of the two Ocimum plants was compared with citronella and DEET to study the repellence and feeding inhibition of untreated and treated arms of volunteers. Using filter papers impregnated with Ocimum extracts, knockdown effects and mortality was investigated on malaria mosquito Anopheles arabiensis and Anopheles gambiae, including a nuisance mosquito, Culex quinquefasciatus. High biting protection (83% to 91%) and feeding inhibition (71.2% to 92.5%) was observed against three species of mosquitoes. Likewise the extracts of Ocimum plants induced KD90 of longer time in mosquitoes than citronella, a standard botanical repellent. Mortality induced by standard dosage of 30 mg/m2 on filter papers, scored after 24 hours was 47.3% for OK and 57% for OS, compared with 67.7% for citronella.
The use of whole plants and their products as insect repellents is common among village communities of north-eastern Tanzania and the results indicate that the use of O. suave and O. kilimandscharicum as a repellent would be beneficial in reducing vector biting. The widespread use of this approach has a potential to complement other control measures.

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    • "In Guinea Bissau, 55% of people burn plants to repel mosquitoes [40]. The result is also comparable to a study reported by Kweka et al., [34]. The use of plant leaves as insect repellent could be one of more sustainable options than any other parts like roots, resin and bark. "
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    ABSTRACT: The usage of insect repellent plants (IRPs) is one of the centuries-old practices in Africa. In Ethiopia, malaria remains a leading cause of morbidity and mortality, subsequently the majority of people have a tendency to apply various plants as repellents to reduce or interrupt the biting activity of insects. Accordingly, this survey was undertaken to document and evaluate knowledge and usage practices of the local inhabitants on IRPs in the malaria epidemic-prone setting of Ethiopia. Ethnobotanical survey was conducted between January and May 2013. Selected 309 household members were interviewed by administering pre-tested questionnaire on knowledge and usage practices of repellent plants, in Bechobore Kebele, Jimma Zone, Ethiopia. Overall, 70.2% (217/309) and 91.8% (199/217) of the respondents have had ample awareness and usage practices of repellent plants, respectively. Informants cited about twenty-two plant species as repellents and also indicated that these plants are useful(85.5%), accessible(86.8%), and affordable(83.9%) too. Residents mainly applying dried leaves [93.9% (187/199)] by means of burning/smouldering [98.9% (197/199)] with the traditional charcoal stove to repel insects, primarily mosquitoes. About 52.8% (105/199) of the informants using aproximately15g of dried plant-materials every day. A Chi-square analysis shows statistically a significant link between the knowledge on repellent plants and gender as well as average monthly income although not with the age of the respondents. Nevertheless, the repellent plant usage custom was not significantly associated with gender, monthly income, and age of the informants. Though most of the people have had an adequate awareness still a sizable faction of society suffers with deprivation of IRPs knowledge and usage practices. Therefore, this study calls for more surveys to conserve the existing indigenous knowledge and cultural practices. It could lay the first stone to develop the next generation cost-effective vector control tools in the near future.
    Full-text · Article · Feb 2014 · Journal of Ethnobiology and Ethnomedicine
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    • "The result of this study also indicated that the majority (73%) of local community members use smoking of the plant part by burning to repel mosquitoes. Similar results have been reported in previous studies carried out in Ethiopia and Tanzania indicating most of the local communities apply smoke to drive away mosquito (Karunamoorthi et al., 2009a; Kweka et al., 2008). In addition, the results also indicated that other types of application such as macerating the plant, laying the leaves of plants inside the house and rubbing onto cloths/skin are important. "
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    ABSTRACT: Insect repellents have been used to reduce human-vector contact for long periods of time in different parts of the world. Thus, the aim of this study was to assess the knowledge of traditional mosquito repellent plants among inhabitants of Kolla Temben district, Ethiopia. Two hundred and forty households were selected for an ethnobotanical survey using stratified, systematic random sampling. One adult leader from each household was interviewed on her or his knowledge about traditional mosquito repellent plants. The ethnobotanical survey was carried out from January-March, 2010. Data analysis was carried out using SPSS, version 16. The level of significance was determined using 95% confidence interval. Ninety-five percent of the respondents had knowledge regarding traditional mosquito repellent plants. Application of smoke by burning (73%) the parts plants was the most common practice among the inhabitants. There was no significant association between knowledge of traditional mosquito repellent plants with age (p = 0.402), sex (p = 0.067) or educational status (p = 0.052) of the respondents. Moreover, the survey revealed that the most commonly known traditional mosquito repellent plants were Otostegia integrifolia (41.7%), Silene macroserene (24.6%,) (Olea europeae (22.5%), Melia azedarach (17.5%), Calpurnia aurea (9.6%), Dodonaea angustifolia (8.7%), Eucalyptus globulus (8.3%), Ere (Aloe spp) 6.7% and Sasa (Otostegia fruticosa) 2.5%. These preliminary findings suggested that the studied community has potentially important knowledge about traditional mosquito repellent plants. Therefore, further study on conserving the knowledge and evaluating the efficacy of the plants must be done.
    Full-text · Article · Jul 2013
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    • "Plant based arthropod repellents currently in the market includes Citronella (Cymbopogonnardus or Cymbopogonwinterianus) [15] and lemon eucalyptus (Eucalyptus maculate citiodon) [16]. Since ancient times, plant products or whole plants have been used to repel or kill mosquitoes and other domestic insect pests in communities [3-6]. The methods of delivering these traditional repellents were thermal expulsion and direct burning, which have been demonstrated to cause reduction in indoor density of mosquitoes [5,6]. "
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    ABSTRACT: The reduction of malaria parasite transmission by preventing human-vector contact is critical in lowering disease transmission and its outcomes. This underscores the need for effective and long lasting arthropod/insect repellents. Despite the reduction in malaria transmission and outcomes in Tanzania, personal protection against mosquito bites is still not well investigated. This study sought to determine the efficacy of menthol propylene glycol carbonate (MR08), Ocimum suave as compared to the gold standard repellent N, N-diethyl-methylbenzamide (DEET), either as a single dose or in combination (blend), both in the laboratory and in the field against Anopheles gambiae s.l and Culex quinquefasciatus. In the laboratory evaluations, repellents were applied on one arm while the other arm of the same individual was treated with a base cream. Each arm was separately exposed in cages with unfed female mosquitoes. Repellents were evaluated either as a single dose or as a blend. Efficacy of each repellent was determined by the number of mosquitoes that landed and fed on treated arms as compared to the control or among them. In the field, evaluations were performed by human landing catches at hourly intervals from 18:00 hr to 01:00 hr. A total of 2,442 mosquitoes were collected during field evaluations, of which 2,376 (97.30%) were An. gambiae s.l while 66 (2.70%) were Cx. quinquefaciatus. MR08 and DEET had comparatively similar protective efficacy ranging from 92% to 100 for both single compound and blends. These findings indicate that MR08 has a similar protective efficacy as DEET for personal protection outside bed nets when used singly and in blends. Because of the personal protection provided by MR08, DEET and blends as topical applicants in laboratory and field situations, these findings suggest that, these repellents could be used efficiently in the community to complement existing tools. Overall, Cx. quinquefasciatus were significantly prevented from blood feeding compared to An. gambiae s.l. The incorporation of these topical repellents for protection against insect bites can be of additional value in the absence or presence of IRS and ITNs coverage. However, a combination of both the physical (bed nets) and the repellent should be used in an integrated manner for maximum protection, especially before going to bed. Additional research is needed to develop repellents with longer duration of protection.
    Full-text · Article · Sep 2012 · Parasites & Vectors
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