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Mitchell PS, Parkin RK, Kroh EM, et al. Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci USA 2008;105:10513-10518

Divisions of Human Biology, Clinical Research, and Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
Proceedings of the National Academy of Sciences (Impact Factor: 9.67). 08/2008; 105(30):10513-8. DOI: 10.1073/pnas.0804549105
Source: PubMed

ABSTRACT

Improved approaches for the detection of common epithelial malignancies are urgently needed to reduce the worldwide morbidity and mortality caused by cancer. MicroRNAs (miRNAs) are small ( approximately 22 nt) regulatory RNAs that are frequently dysregulated in cancer and have shown promise as tissue-based markers for cancer classification and prognostication. We show here that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity. miRNAs originating from human prostate cancer xenografts enter the circulation, are readily measured in plasma, and can robustly distinguish xenografted mice from controls. This concept extends to cancer in humans, where serum levels of miR-141 (a miRNA expressed in prostate cancer) can distinguish patients with prostate cancer from healthy controls. Our results establish the measurement of tumor-derived miRNAs in serum or plasma as an important approach for the blood-based detection of human cancer.

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    • "For miRNAs exported in extracellular vesicles, compelling evidence supports their role in affecting a broad range of physiological and pathological processes. It is believed that these extracellular miRNA-associated unication (Lotvall and Valadi 2007; Valadi et al. 2007; Iguchi et al. 2010; Mathivanan et al. 2010; Vickers et al. 2011) and recent studies have shown that the level and composition of these extracellular/circulating miRNAs correlate well with diseases or injurious conditions (Mitchell et al. 2008; Laterza et al. 2009). A global survey of the miRNA distribution in 12 human body fluids (i.e. "
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    ABSTRACT: The selection of biomarkers in nutrigenomics needs to reflect subtle changes in homoeostasis representing the relation between nutrition and health, or nutrition and disease. It is believed that noncoding RNAs, such as circulating microRNAs (miRNAs), may represent such a new class of integrative biomarkers. Until now, the most relevant body fluids for miRNA quantification in response to nutrition have not been clearly defined, but recent studies listed in this review indicate that miRNAs from plasma or serum, PBMC and faeces might be relevant biomarkers to quantify the physiological impacts of dietary or lifestyle intervention studies. In addition, a number of recent studies also indicate that miRNAs could permit to monitor the impact of diet on gut microbiota. We also discuss the main preanalytical considerations that are important to take into account before miRNA screening which can affect the reproducibility of the data.
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    • "Physiology Cellular Physiology therefore easily accessible and can be used as diagnostic markers for multiple cancers (Mitchell et al., 2008; Kosaka et al., 2010). miRNAs appear to also be passively released by dying cells (Brase et al., 2010), and as a result, circulating miRNA profiling may potentially reflect response to anticancer treatments. "
    Dataset: Article JCP

    Full-text · Dataset · Jun 2015
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    • "secrete specific circulating miRNAs (Pigati et al., 2010) that are therefore easily accessible and can be used as diagnostic markers for multiple cancers (Mitchell et al., 2008; Kosaka et al., 2010). miRNAs appear to also be passively released by dying cells (Brase et al., 2010), and as a result, circulating miRNA profiling may potentially reflect response to anti-cancer treatments. "
    Dataset: jcp24730

    Full-text · Dataset · Jun 2015
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