Preliminary Investigation of Antimicrobial Property of Acacia leucophloea Leaves Extract


Leaves of indigenous plant Acacia leucophloea have been successively extracted using different solvents like hexane, methanol and water. Extract was evaluated for its antibacterial activity using two assay method Kirby bauer disc diffusion and tube dilution assay. Results of the given study revealed that the methanolic extract is potential antibacterial agent specifically for E coli & & S aureus.

Download full-text


Available from: Rajeev K Singla
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Endocarp of C. nucifera L.(Coconut fruit), a universally acceptable waste material is the plant part for our study. Dry distilled extract, RNDS, was prepared using the special procedure adopted by our lab. It was evaluated for its antimicrobial activity using Kirby bauer agar diffusion assay technique. In gram negative- P. aeruginosa & E. coli strains were used and in gram positive – S. aureus & B. subtilis were used, while four fungal strains like A. oryzae, C. albicans, R. oligosporus and A. flavus were part of current study. Results revealed that RNDS is expressing potential growth inhibition of B. subtilis and Aspergillus species. It was inactive against R. oligosporus at all concentrations.
    Full-text · Article · Aug 2012
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: 1,3-oxazolidin-2-one, an oxygen and nitrogen comprising heterocyclic structure attracts many researchers all over world, evidenced to have unique and potential antibacterial activity. We had designed and synthesized few analogues of 1,3-oxazolidin-2-ones. Their structures were characterized using physical and spectral data. To evaluate their antibacterial potential, Kirby Bauer Agar Diffusion Assay procedure was adopted. Results are very significant and revealed that 1,3-oxazolidin-2-ones have potential antibacterial activity when compared with standard Oxytetracycline. In case of E. coli, unsubstituted parent molecule have better inhibitory effect than the substituted ones while in case of P. vulgaris, substituted analogs have higher effect than the parent one.
    Full-text · Article · Sep 2012
  • [Show abstract] [Hide abstract]
    ABSTRACT: The present study was investigated for phytochemical screening and anti-diabetic, antihyperlipidemic, antioxidant activities of Acacia leucophloea in streptozotocin-nicotinamide induced type II diabetes in rats. In the present study thirty rats were randomly classified into five groups. The first group was served as a control. The second group was served as Diabetic control. The third group was served as Standard (Glibenclamide 10mg/kg), fourth group was served as test 1 administered with 200 mg/kg and fifth group was served as test 2 administered with 400 mg/kg of Ethanolic extracts of Acacia leucophloea stem bark in a daily oral dose for 14 days. Type II diabetes was induced in overnight fasted rats by a single intraperitonial injection of 60mg/kg streptozotocin, 15 min after the i.p. administration of 120mg/kg body weight of nicotinamide. Hyperglycemia was confirmed by the elevated glucose levels in plasma, determined at 72 h and then on day 7 after injection of inducing agents. Treatment of animals with extracts of Acacia leucophloea in a daily dose of 200 and 400 mg/kg for 14 consecutive days significantly mitigated the induced changes in the glucose, lipid profile, oxidative stress, liver enzymes and body weight parameters. The induced alterations in the oxidant and antioxidant parameters were also improved. Conclusively, ethanolic extract of Acacia leucophloea stem bark treatment exhibited marked beneficial effects against streptozotocin-nicotinamide induced diabetes.
    No preview · Article · Jan 2014 · Global Journal of Pharmacology