H2O2 Determination by the I3- Method and by KMnO4 Titration

Analytical Chemistry (Impact Factor: 5.64). 09/1994; 66(18). DOI: 10.1021/ac00090a020


The analysis of aqueous H2O2 at concentrations as low as 1 mu M is conveniently done by the I-3(-) method, which is based on the spectrophotometric determination of I-3(-) formed when H2O2 is added to a concentrated solution of I-. At 351 nm, epsilon(max) (I-3(-)) was measured to be 26 450 M(-1) cm(-1). By contrast, an apparent value of 25 800 M(-1) cm(-1) was determined from a calibration of the I-3(-) method against titration by permanganate. The difference could only be partially accounted for by the equilibrium between I-3(-), I-2, and I-. A further correction of similar to 1% was required and was traced to a side reaction between H2O2 and the buffer normally used in the I-3(-) method. A simple spectrophotometric procedure was developed which improves the sensitivity of the permanganate titration to 0.3 mu M H2O2. Measurements of H2O2 using the oxidation of ferrous ions (Fricke solution) and permanganate titration differed by less than 1%.

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    • "The rapid, accurate and reliable detection of hydrogen peroxide is of great significance in many fields such as clinical treatment [2], food and environment safety [3], chemical reactions [4], and pharmaceutical applications [5]. In the past few decades, many analytical methods for H 2 O 2 detection have been developed including spectrometry [6], titration [7], fluorescence [8], chemiluminescence [9], and chromatographic techniques [10]. However, these methods are usually expensive, time-consuming, and require professional personnel, which make them less favorable for rapid and cost-effective detection of H 2 O 2 . "
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    ABSTRACT: In this research, different CuO nanostructures (heart/dumbbell-like and grass-like) were successfully synthesized via simple hydrothermal reactions at 130◦C with different amounts of Cu(NO3)2·2.5H2O in 20 mL H2O and 12 mL NH3·H2O for 6 h in the absence of any additive. The initial amount of Cu(NO3)2·2.5H2O was found to be critical for CuO morphology evolution. In addition to morphology study by scanning electron microscopy (SEM) and crystal structure study by X-ray diffraction (XRD), as-synthesized samples were characterized systematically by electrochemical methods including cyclic voltammetry (CV), amperometric detection (i–t) and electrochemical impedance spectroscopy (EIS). It was found that both heart/dumbbell-like and grass-like CuO nanostructures exhibited good electrochemical performance toward low concentrations of H2O2. High sensitivity, fast and linear response were achieved, which was mainly due to their large specific surface areas and efficient electron transport in corresponding reactions, making them promising candidates for efficient and precise non-enzymatic detection of H2O2.
    Full-text · Article · Mar 2015 · Sensors and Actuators B Chemical
    • "The reliable, accurate and fast detection of hydrogen peroxide (H 2 O 2 ) is of great significance, since H 2 O 2 is a common oxidizing agent and an essential intermediate in biomedical, pharmaceutical , industrial and environmental fields and enzymatic reactions [1]. Among various techniques for H 2 O 2 detection [2] [3] [4] [5] [6] [7], electrochemical method is attractive due to its high sensitivity, simple operation, low cost, and the possibility for real-time detection [8] [9]. "
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    • "All spectrophotometric measurements (e.g., UV 254 , [H 2 O 2 ]) were carried out using a UVeVis spectrophotometer (Shimadzu UV-Mini 1240) with a cell path length of 1 cm. The concentration of H 2 O 2 was measured (detection limit of 1 mM of H 2 O 2 ) using the triiodide method (Klassen et al., 1994). The portion of NOM that absorbs UV at 254 nm was defined as chromophoric NOM (CNOM) (Sarathy et al., 2011). "
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