Identification of alg3 in the mushroom-forming fungus Schizophyllum commune and analysis of the Δalg3 knockout mutant
Institute of Cell Biology and Plant Biochemistry, University of Regensburg, 93053 Regensburg, Germany. Glycobiology
(Impact Factor: 3.15).
09/2012; 23(2). DOI: 10.1093/glycob/cws135
Alg3 of Saccharomyces cerevisiae catalyzes the mannosyl transfer from Man-P-Dol to Man(5)GlcNAc(2)-PP-Dol resulting in the formation of Man(6)GlcNAc(2)-PP-Dol, which is then further processed to the final precursor oligosaccharide Glc(3)Man(9)GlcNAc(2) for N-glycosylation of proteins. Here, we identified the alg3 gene of the mushroom forming fungus Schizophyllum commune by homology search. Its function was confirmed by complementation of the Δalg3 strain of S. cerevisiae. Inactivation of alg3 in S. commune resulted in production of predominantly Man(3)GlcNAc(2) protein-linked N-glycans. No impact on growth nor a developmental phenotype due to the deletion was observed. This provides a first step towards engineering of a homogeneous, humanized N-glycosylation pattern for the production of therapeutic glycoproteins in mushrooms.
Available from: Ya-Jie Tang
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ABSTRACT: Higher fungi or called as macro-fungi, consisting of the divisions ascomycetes, basidiomycetes, and imperfect fungi, are receiving great interest around the world, because studies of higher fungi help us not only to find new edible and officinal resources but also to understand their complicated biology. In recent decades, a large number of useful substances from higher fungi have been isolated, identified, and characterized, which have important biological functions, such as reducing blood pressure, enhancing immunity, and possessing anti-cancer and anti-HIV and other pharmacological activities. This chapter will review the genetic manipulation tools for higher fungi, omics analysis of higher-fungus cell factories, and production of useful metabolites by higher fungi, including those of terpenoids, heterocyclics, polysaccharides, and polyketides. Trends in future development of cell factories of higher fungi for useful metabolite production will also be analyzed.
Strategies for improving cell factories of higher fungi for useful metabolite production
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