Qualité hygiénique du lait de colportage prélevé des points de vente de la ville de Rabat / Hygienic quality of collected hawking milk outlets in the city of Rabat

Les Technologies de Laboratoire 01/2012; 7.


Des échantillons de lait cru, prélevés des points de vente de la ville de Rabat, ont été analysés en vue d'évaluer leur qualité hygiénique. La flore aérobie mésophile totale était élevée dans le lait cru de colportage (4.4 107UFC/mL). La charge moyenne en coliformes totaux et fécaux était de 3.8 105UFC/mL et 1.91 105UFC/mL . Les staphylocoques étaient absents dans tous les échantillons. La présence d’une flore de contamination (Klebsiella, Proteus mirabilis et E. coli) témoignent d’un manque d'hygiène au cours du circuit de colportage du lait cru. L'isolement et l'identification biochimique des 9 souches de bactéries lactiques a mis en évidence 3 espèces du genre Lactobacillus : Lactobacillus acidophilus, Lactobacillus amylovorus et Lactobacillus yamanashiensis.

Samples of raw milk were collected from retail outlets in the city of Rabat, were analyzed to assess the hygienic quality. Results indicated that counts of the aerobic plate count were particularly high in all the samples analyzed (4.4 107CFU/mL). Total and fecal coliforms were average of 3.8 105 CFU/mL and 1.91 105CFU/mL respectively. Staphylococcus were absent in all samples. The presence of germ contamination (Klebsiella, Proteus mirabilis and E coli) attest the poor hygienic quality of circuit hawking milk. Isolation and biochemical identification of nine strains of lactic bacteria revealed three species of Lactobacillus genus: Lactobacillus acidophilus, Lactobacillus amylovorus et Lactobacillus yamanashiensis.

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    ABSTRACT: This paper aims to study the overall quality of raw milk in the mid-northern region of Algeria. The analysis results showed a decrease in the average temperature for the delivery of 1,54°C with P<0.001. However, no significant variation (P>0.05) was observed in almost all the physical and nutritional parameters studied (pH, fat content, and protein content) between M1 and M2. The average contamination by total mesophilic aerobic bacteria (TMAB), coliforms, yeasts, molds, and different pathogens in samples taken at M1 showed significant changes at M2. This was confirmed by the decrease of reduction time of methylene blue (RTMB), about 54%. The variation was described as follows: (P>0.05) for yeasts and (P<0.05) for molds in M1 and M2, (P<0.05) for TMAB in M1, and (P<0.001) for TC, FC, and TMAB in M2. The analysis for the detection of Salmonella spp. showed no contamination in all samples tested, while antibiotic residues were detected in 35% of milks delivered. In conclusion, several risk factors have been identified in this study, namely, the effect of the season and the distance between the farm and the dairy unit.
    Full-text · Article · Oct 2014