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[Show abstract][Hide abstract] ABSTRACT: Spinach chloroplast membranes and aqueous dispersions of their extracted lipids have been studied by spin label (stearic acid)
electron spin resonance and carbon-13 nuclear magnetic resonance techniques. Combined with electron microscope studies, first
systematic evidence is found for the existence of a dynamic lipid-bilayer structure in the chloroplast membranes.
Full-text · Article · Dec 1990 · Journal of Biosciences
[Show abstract][Hide abstract] ABSTRACT: A novel approach to carbon-13 (13C) enrichment of chloroplast membranes (and plant materials in general) is presented for 13C-nuclear magnetic resonance (13C-NMR) studies. The method minimizes the occurrence of spectral complications arising from 13C-13C couplings resulting from a statistical distribution of 13C within the molecule with low probability of encountering two 13C atoms adjacent to each other. This is achieved by growing the plants in light surrounded by an atmosphere containing 1/3rd 12CO2 and 2/3rd 13CO2, liberated by weak acid-treatment of a mixture of corresponding barium carbonate salts.
Full-text · Article · Jan 1988 · Journal of Biochemical and Biophysical Methods
[Show abstract][Hide abstract] ABSTRACT: A new and simple approach for the determination of the temperature of gel-to-liquid crystalline phase transitions (Tc) of biological (chloroplast) membrane lipids from 13C-NMR resonance intensities is proposed. The variation of intensity of a temperature-sensitive NMR resonance is monitored by recording the spectra of the sample at a range of temperatures. From such a series of spectra recorded at different temperatures, a temperature-insensitive resonance is located. Then the ratio of the intensity of the temperature-sensitive to the intensity of the temperature-insensitive resonance is calculated from each spectrum to even out the procedural error, if any. The values of this ratio at different temperatures, when plotted against sample temperature, shows a break at Tc as confirmed by spin label ESR studies.
Full-text · Article · Apr 1990 · Journal of Biochemical and Biophysical Methods