Antimicrobial and Enzyme Activity of Endophytic Fungi Isolated from Tulsi



A study was carried out to isolate endophytic fungi from Ocimum species (Tulsi) and to assess their antimicrobial activity against Pseudomonas aeroginosa, Mycobacterium smegmatis, Salmonella typhimurium, Candida albicans and Penicillium chrysogenum and production of enzymes such as amylase, protease and tyrosinase. In this study forty endophytic fungi were isolated from leaves and branches of Tulsi. Out of forty endophytic fungal isolates, six isolates showed the inhibition activity against test microorganisms done by dual culture method. The isolate P14T1 exhibited a high antimicrobial activity against Candida albicans (22mm). The crude extract of P13T5 isolate showed highest zone of inhibition against Pseudomonas aeroginosa (21mm) by well and disc diffusion method. 50% of fungal isolates found positive for amylase and protease enzymes production and 27.5% showed positive for tyrosinase. The results of the study suggest that endophytic fungi associated with Tulsi are potential agents for antimicrobial activity and production of enzyme.

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    • "Extraction of secondary metabolites, fungi were grown on 500ml of Erlynmeyer flask containing potato dextrose broth for 21 days. Presence of metabolites in the culture filtrate synthesized by the fungi was extracted by addition of equal amount of ethyl acetate [14]. Culture filtrates with the ethyl acetate were transferred to separating funnel and then it was kept for 24hrs for extraction of metabolites, intermediate shaking of the culture filtrate with solvent enhances the better extraction of compounds. "
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