Force-Generation Capacity of Single Vastus Lateralis Muscle Fibers and Physical Function Decline With Age in African Green Vervet Monkeys

Department of Internal Medicine, Gerontology, Wake Forest School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157. .
The Journals of Gerontology Series A Biological Sciences and Medical Sciences (Impact Factor: 5.42). 08/2012; 68(3). DOI: 10.1093/gerona/gls143
Source: PubMed


Previous studies on the contractile properties of human myofibrils reported increase, decrease, or no change with aging, perhaps due to the differences in physical activity, diet, and other factors. This study examined physical performance and contractile characteristics of myofibrils of vastus lateralis (VL) muscle in young adult and old African green vervet monkeys. Animals were offered the same diet and lived in the same enclosures during development, so we were able to examine skeletal muscle function in vivo and in vitro with fewer potential confounding factors than are typical in human research studies. Fiber atrophy alone did not account for the age-related differences in specific force and maximal power output. Regression modeling used to identify factors contributing to lower fiber force revealed that age is the strongest predictor. Our results support a detrimental effect of aging on the intrinsic force and power generation of myofilament lattice and physical performance in vervet monkeys.

4 Reads
  • Source
    • "Type II Species Author Mass (kg) Muscle Type I A B X Hybrid Method Human (Homo sapiens) Green et al., 1981 79.1 VL 46.3 44.3 8.9 ATPase GM 49.4 42.8 6.6 Chimpanzee (Pan troglodytes) a Myatt et al., 2011 56/62 GM 14/16 83/82 MAb Orangutan (Pongo abelii) Myatt et al., 2011 42 GM 47 51 MAb Rhesus macaque (Macaca mulatta) Fitts et al., 1998 9.4 GM 23 24 49 4 MAb Jouffroy et al., 1999 5–6 VL 15 85 MAb GM 22 78 Green vervet monkey (Chlorocebus aethiops sabaeus) Choi et al., 2012 5.6 VL 79 21 SDS-PAGE Feng et al., 2012 5.6 VL 6 78 16 ATPase Marmoset (Callithrix jacchus) Present study 0.34 VL Dist 0.1 68.9 31.0 MAb VL Prox 1.1 56.5 42.4 GM Dist 8.6 48.0 43.4 GM Prox 9.7 40.6 49.7 Bushbaby (Galago senegalensis) Ariano et al., 1973 0.25–0.31 "
    [Show abstract] [Hide abstract]
    ABSTRACT: The muscle mass-specific mean power output (P MMS,mean) during push-off in jumping in marmosets (Callithrix jacchus) is more than twice that in humans. In the present study it was tested whether this is attributable to differences in muscle contractile properties. In biopsies of marmoset m. vastus lateralis (VL) and m. gastrocnemius medialis (GM) (N=4), fibre-type distribution was assessed using fluorescent immunohistochemistry. In single fibres from four marmoset and nine human VL biopsies, the force–velocity characteristics were determined. Marmoset VL contained almost exclusively fast muscle fibres (>99.0%), of which 63% were type IIB and 37% were hybrid fibres, fibres containing multiple myosin heavy chains. GM contained 9% type I fibres, 44% type IIB and 47% hybrid muscle fibres. The proportions of fast muscle fibres in marmoset VL and GM were substantially larger than those reported in the corresponding human muscles. The curvature of the force–velocity relationships of marmoset type IIB and hybrid fibres was substantially flatter than that of human type I, IIA, IIX and hybrid fibres, resulting in substantially higher muscle fibre mass-specific peak power (P FMS,peak). Muscle mass-specific peak power output (P MMS,peak) values of marmoset whole VL and GM, estimated from their fibre-type distributions and force– velocity characteristics, were more than twice the estimates for the corresponding human muscles. As the relative difference in estimated P MMS,peak between marmosets and humans is similar to that of P MMS, mean during push-off in jumping, it is likely that the difference in in vivo mechanical output between humans and marmosets is attributable to differences in muscle contractile properties.
    Full-text · Article · May 2015 · Journal of Experimental Biology
  • Source
    • "Unlike the miR-1 family which is organized as bicistronic genes (Kusakabe et al., 2013), miR-208b and miR-499 are encoded within the slow myosin heavy chain (MHC) genes (van Rooij et al., 2009) and thereby are restricted to type I fibers and modulate fiber type (Endo et al., 2013; McCarthy et al., 2009; van Rooij et al., 2009). A transition from fast-to slow-twitch fibers has been reported in aging rodents and humans (Andersen, 2003; Lexell, 1995), and type-I fibers largely outnumber type-II fibers in our samples from monkey and human vastus lateralis muscles (Choi et al., 2013; Zhang et al., 2014). Thus, changes in miR-208b and miR-499 are intriguing and particularly relevant for muscle fiber function. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Regular exercise, particularly resistance training (RT), is the only therapy known to consistently improve muscle strength and quality (force per unit of mass) in older persons, but there is considerable variability in responsiveness to training. Identifying sensitive diagnostic biomarkers of responsiveness to RT may inform the design of a more efficient exercise regimen to improve muscle strength in older adults. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression. We quantified six muscle specific miRNAs (miR-1, -133a, -133b, -206, -208b and -499) in both muscle tissue and blood plasma, and their relationship with knee extensor strength in seven older (age=70.5±2.5years) adults before and after 5months of RT. MiRNAs differentially responded to RT; muscle miR-133b decreased, while all plasma miRNAs tended to increase. Percent changes in knee extensor strength with RT showed strong positive correlations with percent changes in muscle miR-133a, -133b, and -206 and with percent changes in plasma and plasma/muscle miR-499 ratio. Baseline level of plasma or plasma/muscle miR-499 ratio further predicts muscle response to RT, while changes in muscle miR-133a, -133b, and -206 may correlate with muscle TNNT1 gene alternative splicing in response to RT. Our results indicate that RT alters muscle specific miRNAs in muscle and plasma, and that these changes account for some of the variation in strength responses to RT in older adults. Copyright © 2014. Published by Elsevier Inc.
    Full-text · Article · Jan 2015 · Experimental Gerontology
  • [Show abstract] [Hide abstract]
    ABSTRACT: Specific alterations in the pulsatility of luteinizing hormone (LH) are linked to obesity-related subfertility in ovulatory women. Vervet monkeys (Chlorocebus aethiops sabaeus) are an Old World nonhuman primate that develops obesity and has a menstrual cycle similar to humans. We evaluated follicular-phase LH pulses in 12 adult normal-weight female vervets. Serum was collected every 10 min for 4 h by using a tether device in conscious, freely moving monkeys on menstrual cycle days 2 through 5. Serum estradiol was collected daily during the follicular phase to identify the luteal-follicular transition. For comparison, we used data from 12 ovulatory normal-weight women who had undergone frequent blood sampling of early-follicular LH. LH pulse frequency was similar, with 2.8 ± 0.7 LH pulses during 4 h in vervets compared with 2.3 ± 0.7 LH pulses during 4 h in women. The LH pulse mass (percentage change in the pulse peak over the preceding nadir) was 123.2% ± 27.4% in vervets and 60.9% ± 14.9% in humans. The first day of low serum estradiol after the follicular-phase peak was denoted as the day of the luteal-follicular transition. Luteectomy was performed on luteal days 7 through 9, and corpora lutea were confirmed by histology. We demonstrate that follicular LH patterns in vervets are similar to those in humans and that the luteal phase is easily identified by monitoring daily serum estradiol. These findings demonstrate that vervet monkeys are a suitable animal model for evaluating LH pulse dynamics longitudinally in studies of diet-induced obesity.
    No preview · Article · Oct 2013 · Comparative medicine
Show more