Antidiabetic and antioxidant activities of Toddalia asiatica (L.) Lam. Leaves in Streptozotocin induced diabetic rats
Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai 600034, India. Journal of ethnopharmacology
(Impact Factor: 3).
07/2012; 143(2):515-23. DOI: 10.1016/j.jep.2012.07.006
The leaves of Toddalia asiatica (L.) Lam. have been utilized traditionally for the cure of diabetes.
The present study was aimed to assess the antidiabetic and antioxidant effects of T. asiatica leaves in Streptozotocin (STZ) induced diabetic rats.
The phytochemical screening, total phenolic content, HPLC analysis, acute toxicity study and oral glucose tolerance test were carried out. Glucose lowering effect of the hexane, ethyl acetate and methanol extracts of T. asiatica leaves was studied in STZ-induced diabetic rats. The antidiabetic and antioxidant activities were studied for the ethyl acetate extract. The effects of extracts on blood glucose, body weight, plasma insulin, total protein, liver glycogen, plasma enzymes (SGOT, SGPT and ALP) and activities of SOD, CAT and GPx were analyzed.
T. asiatica leaves ethyl acetate extract (TALEe) showed highly significant blood glucose lowering effect. Phytochemical evaluation of TALEe showed the presence of alkaloids, terpenoids, cumarins, flavonoids and phenolic compounds. The total phenolic content of TALEe was 126mg of gallic acid equivalents/g extract. HPLC analysis revealed the presence of flindersine and ulopterol. Acute toxicity study of TALEe revealed no death or toxicity. The oral glucose tolerance test showed lowered area under curve (AUC(glucose)) values in TALEe treated rats. After treatment with TALEe (250 and 500mg/kg) for 28 days there was a significant decrease in blood glucose, plasma enzymes (SGOT, SGPT and ALP) and significant increase in body weight, total protein, serum insulin and liver glycogen levels in treated diabetic rats. The activities of antioxidant enzymes SOD, CAT and GPx were reversed to near normal in treated diabetic rats. Histopathology of pancreas in TALEe treated groups showed regeneration of β-cells.
The results of the experiments showed that TALEe exerted significant antidiabetic and antioxidant effects in STZ-induced diabetic rats justifying its traditional use.
Available from: Hairin Taha
- "There is a growing research interest in the management of diabetes mellitus (DM) and its associated complications due to the unabated increase in the incidence of this metabolic syndrome. At present DM is one of the alarming global threat giving rise to complications such as retinopathy, neuropathy, heart attack and atherosclerotic vascular disease (Gandhi et al., 2014; Irudayaraj et al., 2012; Laakso, 1999). The impairment in carbohydrate, lipid "
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ABSTRACT: Ethnopharmacological relevance:
The art of Ayurveda and the traditional healing system in India have reflected the ethnomedicinal importance of the plant Woodfordia fruticosa Kurtz, which demonstrates its vast usage in the Ayurvedic preparations as well as in the management of diabetes by the traditional healers of Beed district in Maharashtra.
Aims of study:
The study aimed to ascertain the antidiabetic potential of Woodfordia fruticosa flower methanolic extract (WF) on Streptozotocin (STZ)-nicotinamide-induced diabetic rat model.
Materials and methods:
Diabetes was induced in Sprague Dawley (SD) male rats by STZ-nicotinamide and the diabetic rats were treated with three different doses of WF (100, 200 and 400mg/kg body weight) and glibenclamide as a positive control. Biochemical parameters such as blood glucose, serum insulin and C-peptide levels were measured with oxidative stress markers. Moreover, histology of liver and pancreas were carried out to evaluate glycogen content and β-cells structures, thereafter immunohistochemistry and western blot analysis were carried out on kidney and pancreas tissues to determine renal Bcl-2, pancreatic insulin and glucose transporter (GLUT-2, 4) proteins expression in all the experimental groups.
The acute toxicity study confirmed the non-toxic nature of all the three doses of WF and further study models revealed the anti-hyperglycemic effects of WF by upregulating serum insulin and C-peptide levels in the diabetic rats. Similarly, WF shown to ameliorate oxidative stress by downregulating LPO levels and augmenting the antioxidant enzyme (ABTS). Furthermore, histopathological analysis demonstrate recovery in the structural degeneration of β-cells mass of pancreas tissue with increase in the liver glycogen content of the diabetic rats. The protective nature of the extract was further confirmed by the immunohistochemical study result which displayed upregulation in the insulin and renal Bcl-2 expression, the anti apoptosis protein. Moreover, the western blot result shown slight alteration in the GLUT-2 and GLUT-4 protein expression with the highest dosage of WFc treatment, this might have stimulated glucose uptake in the pancreas and played an important role in attenuating the blood glucose levels.
The data of the present study revealed the potential of WF in the management of diabetes and its related complications, thus warrants further investigation on its major compounds with in depth mechanistic studies at molecular level.
Available from: Ganesh Mahendran
- "This could be due to the result of improved glycemic control exerted by 1 and 2. Reduction in total protein content was observed in diabetic rats which might be due to progressive proteinuria (Latha and Daisy 2011). In experimental diabetes, distinct metabolic renal alterations are demonstrable, leading to a negative nitrogen balance, enhanced proteolysis and lowered protein synthesis (Irudayaraj et al. 2012). The plasma protein level was improved in diabetic rats after treatment with test compounds 1 and 2. Liver is the vital organ of metabolism, detoxification, storage and excretion of xenobiotics and their metabolites. "
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ABSTRACT: 1, 2, 8-trihydroxy-6-methoxy xanthone (1) and 1, 2- dihydroxy-6-methoxyxanthone-8-O-β-d-xylopyranosyl (2) are the main constituents of petroleum ether and ethyl acetate extracts from Swertia corymbosa (Gentinaceae), a medicinal plant used in Indian traditional system for the treatment of diabetes. The present study was designed to examine the antihypoglycemic, antihyperlipidemic and antioxidant effect of compounds 1 and 2 in streptozotocin (STZ) induced diabetic rats. Diabetes was induced in male Wistar rats by a single intraperitoneal injection of STZ (60mg/kgb.w.). The isolated compounds 1 and 2 at a dose of 50mg/kgb.w., produced the maximum fall of 83% in the blood glucose level in the diabetic rats after 3h of the treatment. The administration of 1 and 2 (50mg/kgb.w.) daily for 28 days in STZ induced diabetic rats, resulted in a significant decrease in blood glucose, glycosylated hemoglobin, SGOT, SGPT, ALP serum urea and creatinine with significant rise in plasma insulin level. Test compounds 1 and 2 showed antihyperlipidemic activities as evidenced by significant decrease in serum TC, TG, LDL-C, VLDL-C levels coupled together with elevation of HDL-C level in diabetic treated rats when compared to diabetic untreated rats, indicate the protective role against liver and kidney damage. The results of histopathology also showed 1 and 2 protected tissues (pancreas, liver and kidney) against peroxidation damage and maintained tissue integrity. Further, the molecular interaction study of the ligands 1, 2 and glibenclamide with various diabetes mellitus related protein targets like glucokinase (PDB ID: 1V4S), fructose-1, 6-bisphosphatase 1 (PDB ID: 2JJK) 11-β-hydroxysteroid dehydrogenase (PDB ID: 2BEL) and modeled protein sulfonylurea receptor 1 (SUR1) showed that ligand 1 and 2 possess binding affinity with all protein targets except for 2BEL target protein for which ligand 1 has no interaction. The ligand pose with 2BEL and SUR1 protein target of ligand 2 gave the best binding conformation. Hence 1 and 2 can be considered for developing into a potent antidiabetic drug.
Available from: Vikas Kumar
- "Hyperglycaemia an associate with hyperlipidemia in the late phase of life is also prone to diabetes . It is the complex metabolic endocrine disorder which resulting from disequilibrium of dynamic expression, causing the macro (heart attack, stroke and peripheral vascular disease) and micro (retinopathy, neuropathy and nephropathy) vascular complications [7,8]. Further, increase in the blood glucose level in diabetic patient causes alteration in utilization of glucose in the vital organs viz. "
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ABSTRACT: The primary objective of the present investigation is to evaluate the antidiabetic, antihyperlidemic and antioxidant activity of the methanolic extract of the Paederia foetida Linn. (PF) leaf extract in the streptozotocin induced diabetic rats.
Single intraperitoneal injection (IP) of streptozotocin (60 mg/kg body weight) was used for induction of diabetes is swiss albino (wistar strain) rats. The induction of diabetes was confirmed after 3 days as noticing the increase in blood sugar level of tested rats. PF at a once a daily dose of 100 mg/kg, 250 mg/kg, 500 mg/kg, p.o. alongwith glibenclamide 10 mg/kg, p.o. was also given for 28 days. On the 28th day rats from all the groups fasted overnight fasted and the blood was collected from the puncturing the retro orbit of the eye under mild anesthetic condition. There collected blood sample was used to determine the antihyperlipidemic, hypoglycemic and antioxidant parameters.
The oral acute toxicity studies did not show any toxic effect till the dose at 2000 mg/kg. While oral glucose tolerance test showed better glucose tolerance in tested rats. The statistical data indicated that the different dose of the PF significantly increased the body weight, hexokinase, plasma insulin, high density lipoprotein cholesterol, superoxide dismutase, catalase and glutathione peroxides. It also decreases the level of fasting blood glucose, total cholesterol, triglycerides, low density lipoprotein cholesterol, very low density lipoprotein cholesterol, malonaldehyde, glucose-6-phosphate, fructose-1-6-biphosphate and glycated hemoglobin in STZ induced diabetic rats. The histopathology of STZ induce diabetic rats, as expected the test dose of PF extract considerably modulates the pathological condition of various vital organ viz. heart, kidney, liver, pancreas as shown in the histopathology examinations.
our investigation has clearly indicated that the leaf extract of Paederia foetida Linn. showed remarkable antihyperglycemic activity due to its possible systematic effect involving in the pancreatic and extra pancreatic mechanism. Forever, the antihyperlipidemic activity was exerted possible by lowering the higher level of lipid profile and decreasing the intercalated disc space in the heart. The antioxidant activity of extract was due to inhibition of lipid peroxidation and increasing the SOD, GPx and CAT. It was corroborate that the extract shown the Paederia foetida Linn leaves potential to be act as antidiabetic, antihyperlipidemic and antioxidant properties.
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