Individual Differences in Voluntary Ethanol Consumption Lead to Differential Activation of the Central Amygdala in Rats: Relationship to the Anxiolytic and Stimulant Effects of Low Dose Ethanol

Department of Pharmacology, Physiology and Neuroscience (ACS, KFK, JRF, MAW), School of Medicine, University of South Carolina, Columbia, South Carolina.
Alcoholism Clinical and Experimental Research (Impact Factor: 3.21). 07/2012; 37(s1). DOI: 10.1111/j.1530-0277.2012.01907.x
Source: PubMed


Although alcohol use disorders and anxiety disorders are highly comorbid, the relationship between these 2 disorders is not fully understood. Previous work from our laboratory shows that anxiety-like behavior is highly variable in outbred Long-Evans rats and is related to the level of voluntary ethanol (EtOH) consumption, suggesting that basal anxiety state influences EtOH intake. To further examine the relationship between the acquisition of EtOH consumption and anxiety phenotype, Long-Evans rats were assessed for anxiety-like behavior and neuronal activation following voluntary EtOH consumption in a limited access drinking paradigm.
Rats were allowed to self-administer EtOH (6% v/v) for 4 days using a limited access drinking in the dark paradigm and divided into high- and low-drinking groups based on a median split of average daily EtOH intake. Immediately following the fourth drinking session, animals were tested on the elevated plus maze and evaluated for anxiety-like behaviors. Fos immunoreactivity was assessed in the central and basolateral amygdala, as well as the bed nucleus of the stria terminalis.
High EtOH drinkers spent significantly more time on the open arms of the plus maze than low EtOH drinkers. High EtOH drinkers also had increased locomotor activity as compared to both low EtOH drinkers and water drinkers. Fos immunoreactivity was positively correlated with EtOH consumption in all brain regions examined, although Fos-positive cell counts were only significantly different between high and low EtOH drinkers in the central amygdala (CeA).
Our findings demonstrate that outbred rats will voluntarily consume behaviorally effective doses of EtOH in a short-term access model and EtOH consumption is positively correlated with increased neuronal activation in the CeA.

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    • "Individual differences in alcohol use have been documented in human and preclinical studies (Chassin et al., 2002; Goudriaan et al., 2007; Hayton et al., 2012; Hwa et al., 2011; Sabino et al., 2013; Simms et al., 2008) and several rodent lines have been bred for their differences in alcohol consumption (Colombo et al., 1995; Crabbe et al., 2009; Le et al., 2001; Li and McBride, 1995; Sinclair et al., 1989). For example, studies have examined whether individual differences in anxiety-related behaviors in outbred populations predict high alcohol consumption or vice versa (Bahi, 2013; Hayton et al., 2012; Sharko et al., 2013; Spanagel et al., 1995). However, individual differences in alcohol consumption have not been related to individual differences in alcohol reinforcement and AUD-like behavior in outbred rodents. "
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