Untersuchungen zum Einsatz von Progesteronschnelltests (Serozyme®‐Progesteron, Ovucheck®) zur Graviditätsdiagnose beim österreichischen Bergschaf
Journal of Veterinary Medicine Series A (Impact Factor: 0.93). 05/2010; 39(1‐10):525 - 530. DOI: 10.1111/j.1439-0442.1992.tb00212.x
Investigations Concerning the Use of Progesterone Tests (Serozyme®-Progesterone, Ovucheck®) for Pregnancy Diagnosis of Mountain SheepTo determine the oestrous cycle length of mountain sheep 10 ewes were stimulated with intravaginal sponges (Chronogest®) and ten with prostaglandines (Iliren®) and a PMSG injection (500IU), respectively. Independent of the synchronisation mode the Serozyme®-progesterone levels indicated a cycle length of 17 days. Progesterone was not detectable by the test system during oestrous, it reached its maximum on the 10th day (x̄ = 3.9/3.7 ng/ml) and decreased to non-detectable levels again on day 17. For early pregnancy diagnosis the Serozyme®-progesterone as well as the Ovucheck® gave useful results.On day 17 and 19 after mating the progesterone concentration of pregnant ewes remained on the same level as on day 10, whereas barren ewes had non-detectable progesterone levels on day 17 and 19, using the Serozyme®-progesterone and below 1 ng/ml on day 19 using the Ovucheck®. The accuracy of the Serozyme®-progesterone referring to the declaration “non pregnant” was 100%, that of the Ovucheck® 37.5% on day 17 and 100% on day 19.The use of both test systems for determining pregnancy of unknown length was examined by collecting blood-samples three times with a five and a seven day interval. Precise results were obtained only with the Serozyme®-progesterone test. At least one of three blood samples of all the barren ewes (n = 8) contained amounts of progesterone beneath the sensitivity of this method. The Ovucheck® results could not help at all to distinguish barren or pregnant ewes with unknown mating data.ZusammenfassungZur Erhebung der Zyklusdauer beim Bergschaf wurden je 10 Tiere mit Chronogest®-Vaginalschwmmchen oder Prostaglandin (Iliren®) und anschließender PMSG-(500 IE)Gabe östrussynchronisiert.Das Serozyme®-Progesteronprofil dokumentierte unabhngig von der Stimulationsart eine Zyklusdauer von 17 Tagen. Die Progesteronwerte lagen whrend des Östrus unter der Empfindlichkeitsgrenze des Meßverfahrens, erreichten am 10. Tag ihr Maximum (x̄ = 3,9, bzw. 3,7ng/ml) und sanken bis zum 17. Tag wieder auf das Ausgangsniveau ab. Für die Frühgravidittsdiagnose lieferte sowohl Serozyme®-Progesteron als auch Ovucheck® brauchbare Befunde. Whrend bei den trchtigen Tieren am 17. und 19. Tag nach der Deckung die Progesteronwerte am Niveau des 10. Tages verblieben, waren sie bei den güsten Schafen im Serozyme®-Verfahren am 17. und 19. Tag unter der Nachweisgrenze (< 0,15 ng/ml), beim Ovucheck® am 19. Tag unter 1 ng/ml. Die Genauigkeit der Aussage „nicht trchtig” betrug für das Serozyme®-Progesteron an beiden Tagen 100 %, beim Ovucheck® am 17. Tag 37,5 %, am 19. Tag 100 %. Die dreimalige Blutentnahme im Abstand von 5 bzw. 7 Tagen zum Progesteronnachweis bei Schafen mit unbekanntem Decktermin erlaubte nur beim Serozyme®-Progesteron eine eindeutige Interpretation. Alle Schafe (n = 8), die mindestens einmal zu den drei Untersuchungsterminen einen Progesteronwert unter der Nachweisgrenze aufwiesen, waren güst. Mit der Ovucheck®-Messung war eine Unterscheidung zwischen graviden und güsten Tieren nicht möglich.
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ABSTRACT: Daily transrectal ultrasonographies were conducted to study development of all follicles with antral diameters > or = 2mm during the follicular phase of oestrous cycle in Mouflon, a strictly monovular wild-sheep. A total of 14 follicular phases was studied after oestrus synchronization with two cloprostenol doses, 9 days apart, in five cyclic Mouflon ewes. In 13 cycles (92.8%), the ovulatory follicle arose from those antral follicles present in both ovaries when luteolysis was induced, being the largest one with a mean size of 4.4+/- 0.3mm at that moment in 10 cycles (76.9%). The remaining cycles had a larger follicle, but it was decreasing in size. Appearance of new follicles > or =2mm in size remained unaffected during the follicular phase (3.7+/- 0.2), but there was found a linear decrease in the number of those growing to > or =3mm (2.5+/- 0.4 to 1.1+/- 0.2, P < 0.05) and > or = 4mm (0.6+/- 0.2 to 0.1+/- 0.1, P < 0.005), detection of new follicles growing to > or = 5mm was negligible. Then, number of medium (4-5mm) growing follicles present in both ovaries decreased from 1.5+/- 0.3 at 0 h to 0.3+/- 0.1 at 72 h (P<0.005). In conclusion, the single ovulatory follicle is the largest growing follicle present in both ovaries at the moment of luteolysis. This follicle is selected to grow and ovulate while development of other follicles is inhibited.
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ABSTRACT: The objective of this study was to determine a value of serum progesterone (P4) concentration, assessed using an enzymeimmunoassay (EIA), for the early distinction between pregnant and non-pregnant ewes and goats. Adult, non-lactating ewes of Chios (n=53), Berrichon (n=30) and Sfakia (n=45) breeds were synchronized during the breeding season with progestagens and gonadotrophins and mated to fertile rams (Experiment I). Adult, lactating goats of Swiss breeds (Alpine and Saanen, n=104) and indigenous Greek breed (n=45) were synchronized during the transitional season with progestagens, PGF2alpha and gonadotrophins. Cervical artificial insemination (AI) with fresh semen was applied once, 42-44 h after sponge removal (Experiment II). Jugular blood samples were collected on day 19 after sponge removal (ewes) or on day 21 after AI (goats) and serum P4 concentration was determined by EIA. Progesterone concentrations >/=1.0, >/=1.5, >/=2.5 and >/=4.0 ng/ml were tested as indicative of pregnancy. Pregnancy diagnosis was verified on birth. In the case of sheep, using a discriminatory level of 2.5 ng/ml, overall accuracy of pregnancy diagnosis was 91.4% and predictive value of negative and positive diagnoses were 98.3 and 85.3%, respectively. In the case of goats, predictive value of negative diagnosis was 95.8 and 94.0% and predictive value of positive diagnosis 71.3 and 71.7%, for 1.5 and 2.5 ng/ml, respectively; overall accuracy was 79.2% using either level. The other discriminatory levels tested did not improve these results. A significant positive correlation was observed between P4 concentration and the number of lambs or kids born, and further analysis indicated that this relationship is not a simple linear function. Based on the results of this study, P4 concentrations of 2.5 ng/ml in the case of ewes and 1.5-2.5 ng/ml in the case of goats, determined with EIA, are proposed as discriminatory levels between pregnant and non-pregnant animals, at an interval of one oestrous cycle after service.
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ABSTRACT: Background The physiological levels of endocrine and metabolic parameters in Slovene autochthonous breeds of sheep are not yet well known, nor are the mechanisms of their adaptability and responses to climate and environmental factors. Therefore, the aim of this study was to evaluate fluctuations of cortisol, insulin and non-esterified fatty acids (NEFA) in growing ewes over an one-year period. Blood samples were collected monthly from 10 yearling Jezersko-Solchava, 10 Bovec and 10 Istrian ewes. Serum cortisol, insulin and NEFA were measured with commercial kits. Results Mean monthly cortisol values fluctuated with low levels in summer and high levels in autumn. Significant peaked cortisol values of 25.69 ± 6.89, 14.67 ± 2.43 and 21.11 ± 7.25 μg/L in Jezersko-Solchava, Bovec and Istrian breed, respectively, were found in September (Bovec breed) and October (Jezersko-Solchava and Istrian breed). Mean monthly insulin values increased during the observation period. The highest levels of 14.60 ± 3.15, 16.03 ± 5.35 and 12.56 ± 2.52 μIU/mL in Jezersko-Solchava, Bovec and Istrian breed, respectively, were observed in the last sample collection in May. NEFA concentrations were found to be low except in some autumn and spring months. The peak values were observed in March for Jezersko-Solchava and Istrian breed (0.60 ± 0.05 and 0.66 ± 0.10 mmol/L), and in April for Bovec breed (0.71 ± 0.11 mmol/L). Conclusions Monthly fluctuations of cortisol, insulin and NEFA were measured in all observed sheep breeds, but between-breed differences in monthly values of examined parameters were insignificant. Significantly increased serum cortisol levels were found in autumn for all breeds and were probably associated with the onset of puberty and low environmental temperature. A gradual increase of insulin level in the examined ewes was in parallel with their growth. Significantly higher NEFA values in spring suggest qualitatively insufficient feed supply during that period.
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