Untersuchungen zum Einsatz von Progesteronschnelltests (Serozyme®‐Progesteron, Ovucheck®) zur Graviditätsdiagnose beim österreichischen Bergschaf

Journal of Veterinary Medicine Series A (Impact Factor: 0.93). 05/2010; 39(1‐10):525 - 530. DOI: 10.1111/j.1439-0442.1992.tb00212.x


Investigations Concerning the Use of Progesterone Tests (Serozyme®-Progesterone, Ovucheck®) for Pregnancy Diagnosis of Mountain SheepTo determine the oestrous cycle length of mountain sheep 10 ewes were stimulated with intravaginal sponges (Chronogest®) and ten with prostaglandines (Iliren®) and a PMSG injection (500IU), respectively. Independent of the synchronisation mode the Serozyme®-progesterone levels indicated a cycle length of 17 days. Progesterone was not detectable by the test system during oestrous, it reached its maximum on the 10th day (x̄ = 3.9/3.7 ng/ml) and decreased to non-detectable levels again on day 17. For early pregnancy diagnosis the Serozyme®-progesterone as well as the Ovucheck® gave useful results.On day 17 and 19 after mating the progesterone concentration of pregnant ewes remained on the same level as on day 10, whereas barren ewes had non-detectable progesterone levels on day 17 and 19, using the Serozyme®-progesterone and below 1 ng/ml on day 19 using the Ovucheck®. The accuracy of the Serozyme®-progesterone referring to the declaration “non pregnant” was 100%, that of the Ovucheck® 37.5% on day 17 and 100% on day 19.The use of both test systems for determining pregnancy of unknown length was examined by collecting blood-samples three times with a five and a seven day interval. Precise results were obtained only with the Serozyme®-progesterone test. At least one of three blood samples of all the barren ewes (n = 8) contained amounts of progesterone beneath the sensitivity of this method. The Ovucheck® results could not help at all to distinguish barren or pregnant ewes with unknown mating data.ZusammenfassungZur Erhebung der Zyklusdauer beim Bergschaf wurden je 10 Tiere mit Chronogest®-Vaginalschwmmchen oder Prostaglandin (Iliren®) und anschließender PMSG-(500 IE)Gabe östrussynchronisiert.Das Serozyme®-Progesteronprofil dokumentierte unabhngig von der Stimulationsart eine Zyklusdauer von 17 Tagen. Die Progesteronwerte lagen whrend des Östrus unter der Empfindlichkeitsgrenze des Meßverfahrens, erreichten am 10. Tag ihr Maximum (x̄ = 3,9, bzw. 3,7ng/ml) und sanken bis zum 17. Tag wieder auf das Ausgangsniveau ab. Für die Frühgravidittsdiagnose lieferte sowohl Serozyme®-Progesteron als auch Ovucheck® brauchbare Befunde. Whrend bei den trchtigen Tieren am 17. und 19. Tag nach der Deckung die Progesteronwerte am Niveau des 10. Tages verblieben, waren sie bei den güsten Schafen im Serozyme®-Verfahren am 17. und 19. Tag unter der Nachweisgrenze (< 0,15 ng/ml), beim Ovucheck® am 19. Tag unter 1 ng/ml. Die Genauigkeit der Aussage „nicht trchtig” betrug für das Serozyme®-Progesteron an beiden Tagen 100 %, beim Ovucheck® am 17. Tag 37,5 %, am 19. Tag 100 %. Die dreimalige Blutentnahme im Abstand von 5 bzw. 7 Tagen zum Progesteronnachweis bei Schafen mit unbekanntem Decktermin erlaubte nur beim Serozyme®-Progesteron eine eindeutige Interpretation. Alle Schafe (n = 8), die mindestens einmal zu den drei Untersuchungsterminen einen Progesteronwert unter der Nachweisgrenze aufwiesen, waren güst. Mit der Ovucheck®-Messung war eine Unterscheidung zwischen graviden und güsten Tieren nicht möglich.

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