Immobilization of Papain on the Mesoporous Molecular Sieve MCM‐48

Beijing University of Chemical Technology, Peping, Beijing, China
Engineering in Life Sciences (Impact Factor: 2.49). 10/2005; 5(5):436 - 441. DOI: 10.1002/elsc.200520094


The immobilization of papain on the mesoporous molecular sieve MCM-48 (with a pore size of 6.2 nm in diameter) with the aid of glutaraldehyde, and the characteristics of this immobilized papain are described. The optimum conditions for immobilization were as follows: 20 mg native free enzyme/g of the MCM-48 and 0.75 % glutaraldehyde, 2 h at 10–20 °C and pH 7.0. Under these optimum conditions for immobilization, the activity yield [%] of the immobilized enzyme was around 70 %. The influence of the pH on the activity of the immobilized enzyme was much lower compared to the free enzyme. The thermostability of the immobilized enzyme, whose half-life was more than 2500 min, was greatly improved and was found to be significantly higher than that of the free enzyme (about 80 min). The immobilized enzyme also showed good operational stability, and the activity of the immobilized enzyme continued to maintain 76.5 % of the initial activity even after a 12-day continuous operation. Moreover, the immobilized enzyme still exhibited good storage stability. From these results, papain immobilized on the MCM-48 with the aid of glutaraldehyde, can be used as a high-performance biocatalyst in biotechnological processing, in particular in industrial and medical applications.

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    • "The supports must be made suitable for the technical applications by maintaining a high level of enzyme activity and preventing a possible leaching out during the reactions [2]. Various techniques had been used to immobilize enzymes, including adsorption and covalent bonding, and several approaches have been made for the preparation of immobilized enzymes for the reason that they have shown several advantages over enzymes in bulk solution [3] [4] [5] [6]. Among these methods, adsorption has been considered as a simple and an economical mechanism for immobilization [7]. "
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