An Oxygen‐Insensitive Reagentless Glucose Biosensor Based on Osmium‐Complex Modified Polypyrrole

Ruhr-Universität Bochum, Bochum, North Rhine-Westphalia, Germany
Electroanalysis (Impact Factor: 2.14). 11/2000; 12(17):1383 - 1389. DOI: 10.1002/1521-4109(200011)12:17<1383::AID-ELAN1383>3.0.CO;2-0


An optimized material for the development of reagentless oxygen-independent biosensors based on conducting polymers is described. Considering the prerequisites for a fast electron transfer between a redox enzyme and the electrode surface via an electron-hopping mechanism, an Os-complex-modified pyrrole derivative with a long, flexible spacer chain has been synthesized. Copolymerization of the new mediator-modified pyrrole monomer with pyrrole was optimized aiming on a higher mediator loading in the film. The feasibility of this material for the development of reagentless oxygen-independent biosensors is demonstrated by entrapment of a PQQ-dependent glucose dehydrogenase isolated from Erwinia sp. 34-1 within this electrochemically grown redox-polymer network.

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    • "However, the oxidation of other mono-and disaccharides occur with lower catalytic efficiencies. Therefore, this system could be used for the analysis of a single sugar in different samples where glucose is a major constituent including biological fluids [18] [19]. For the adequate immobilization of an enzyme to an electrode and in order to obtain a substrate- A C C E P T E D M A N U S C R I P T "
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    • "An alternative to the direct electron transfer is the mediator when electrons migrate to or from the oxidized or reduced enzyme active site, respectively, via a low molecular weight redox active molecule with a proper reduction potential. Some mediators have been involved, such as ferroncene and its derivatives [76, 77], phenazine methosulfate [78], benzoquinone [79], N-methylphenazinium [80], Au nanoparticles [81], cytochrome b562 [82], osmium-complexes [83, 84], electroconducting polymers [85] and ruthenium (II/III) complexes. "
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    • "Different types of enzymes were applied in the design of catalytic–electrochemical sensors, but it is evident that the most promising approach for the development of electrochemical biosensors is to establish direct electrical communication between the biomolecules and the electrode surface [2] [3] [4] [5] [6] [7] [8]. Here conducting polymers [3], redox polymers [4] [5] [6] [7] and soluble [7] [8] [9] or covalently attached to enzyme redox mediators [10] were applied as electron transfer shuttles that enable direct amperometric signal detection. Further methodology of amperometric biosensor development involves the application of redox enzymes for the targeted oxidation/reduction of analytes at the electrode supports and the generation of the electrical signal output. "
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