A feasibility study on seeding as a bioremediation practice for the oil Kuwaiti desert

Department of Botany and Microbiology, Faculty of Science, Kuwait University, Kuwait
Journal of Applied Microbiology (Impact Factor: 2.48). 10/2003; 83(3):353 - 358. DOI: 10.1046/j.1365-2672.1997.00237.x


Immediately after a simulated oil spill, and for 28 weeks, Kuwaiti desert samples became steadily enriched with one specific, indigenous, oil-degrading Arthrobacter strain, KCC 201. Other indigenous oil degraders, including other Arthrobacter strains, either remained unchanged at low numbers or steadily disappeared. The partial hydrocarbon degradation in the polluted samples was primarily due to the indigenous, actively propagating Arthrobacter strain. Seeding the 28-week-old polluted samples with local or foreign oil-degrading isolates did not lead to enhancement of hydrocarbon degradation and resulted in dramatic decreases in the numbers of the predominant, indigenous, oil-degrading Arthrobacter strain, KCC 201. Some of the seeded organisms, particularly the foreign isolates, failed to establish themselves in the polluted samples, apparently because of microbial competition.

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    • "Studies in our laboratory on Kuwaiti desert contaminated with oil revealed a rather slow natural recovery of those areas by indigenous hydrocarbon-utilizing microorganisms (Sorkhoh et al., 1992; Radwan et al., 1997). The rhizospheres of wild and crop plants, including legumes, were found to host rich oil-utilizing bacterioflora (Radwan et al., 1998). "
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    ABSTRACT: The surfaces of root nodules of Vicia faba and Lupinus albus (legume crops), were colonized with bacterial consortia which utilized oil and fixed nitrogen. Such combined activities apparently make those periphytic consortia efficient contributors to bioremediation of oily nitrogen-poor desert soils. This was confirmed experimentally in this study. Thus, cultivating V. faba, L. albus and, for comparison, Solanum melongena, a nonlegume crop, separately in oily sand samples resulted in more oil attenuation than in an uncultivated sample. This effect was more pronounced with the legume crops than with the nonlegume crop. Furthermore, in flask cultures, V. faba plants with nodulated roots exhibited a higher potential for oil attenuation in the surrounding water than plants with nodule-free roots. Denaturation gradient gel electrophoresis (DGGE) of polymerase chain reaction amplified 16S rRNA coding genes revealed that periphytic bacteria had DGGE bands not matching those of the oil-utilizing rhizospheric bacteria. Legume nodules also contained endophytic bacteria whose 16S rDNA bands did not match those of Rhizobium nor those of all other individual periphytic and rhizospheric strains. It was concluded that legume crops host on their roots bacterial consortia with a satisfactory potential for oil phytoremediation.
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    • "Addition of microbial nutrients such as carbon, nitrogen, calcium, potassium and magnesium and micronutrient elements such as copper, zinc, cobalt and nickel enhanced biodegradation activities [28] . Seeding practice my be a useful method for bioremediation [30] . Biological degradation is a very effective treatment technology for remediating petroleum contaminated soils. "
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    ABSTRACT: While environmental biotechnology is broadly based in variety of environmental protection, restoration, and agriculture industrial practices, for a decade it has been most commonly linked with bioremediation, rehabilitation and waste treatment technology. Most of the Eastern region of Saudi Arabia, including the coastal area bordering Kuwait, became polluted with petroleum during the recent Gulf war (1990). Microorganisms can degrade petroleum as a carbon and energy source and this might provide scope to remediate and rehabilitate petroleum-contaminated soils. There are several problems dealing with petroleum-contaminated soils which effect human health and environmental quality. The present review may give some opportunities in bioremediation of petroleum-contaminated soils for more future studies in the Arabian Gulf region. تستند التقنية الحيوية بتوسع على مختلف أوجه الحماية البيئية مثل التطبيقات الصناعية والزراعية والبتروكيميائية والهندسة البيئية، وفي العقد الأخير فإن هناك ارتباط وثيق مع الاستصلاح الحيوي وتقنية معالجة التلوث النفطي. تلوثت معظم شواطئ المنطقة الشرقية للمملكة العربية السعودية بالإضافة إلى السواحل المتاخمة للحدود الكويتية بالنفط الخام خلال حرب الخليج الأخيرة عام 1990م. وجد أن الكائنات الحية الدقيقة تستطيع هدم وتفكيك النفط واستخدامه كمصدر للكربون والطاقة وهذا ربما يقدم العديد من الأهداف لمعالجة وتأهيل التربة الملوثة بالنفط ومشتقاته، والتي تؤثر على صحة الانسان وجودة البيئة. الاستعراض الحالي للعديد من الأبحاث العلمية ربما يعطي بعض الفرص في مجال المعالجة والتأهيل الحيوي للتربة الملوثة بالنفط اللمزيد من الدراسات التطبيقية المستقبلية في منطقة الخليج العربي.
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    • "Bioremediation, the removal of hydrocarbons through microbial degradation, is a commonly used approach for decontaminating oilpolluted soils. It has often been recorded that the addition of hydrocarbon-degrading microbes is no more effective at hydrocarbon removal than is stimulating the growth of the indigenous microbiota (Atlas and Atlas 1991; Atlas and Cerniglia 1995), and may even be less effective (Radwan et al. 1997). Consequently, understanding the microbial ecology and the constraints on biodegradation of an oilcontaminated site is extremely important (Holden and Firestone 1997). "
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    ABSTRACT: To study the comparative effect of diesel addition and simulated bioremediation on the microbial community in three different soil types. Three different soils were amended with diesel and bioremediation treatment simulated by addition of nutrients. The progress of bioremediation, and the effect on the indigenous microbial communities, was monitored using microbiological techniques. These included basal respiration, sole carbon source utilization patterns using both a commercially-available substrate set and a set designed to highlight changes in hydrocarbon-utilizing bacteria, and phospholipid fatty acid (PLFA) profiling. The development of active hydrocarbon-degrading communities was indicated by the disappearance of diesel, increases in soil respiration and biomass, and large changes in the sole carbon source utilization patterns and PLFA profiles compared with control soils. However, comparison of the relative community structure of the three soils using PLFA profiling showed that there was no tendency for the community structure of the three different soil types to converge as a result of contamination. In fact, they became more dissimilar as a result. Changes in the sole carbon source utilization patterns using the commercially-available set of carbon sources indicated the same result as shown by PLFA profiling. The specially selected set of carbon sources yielded no additional information compared with the commercially-available set. Diesel contamination does not result in the development of similar community profiles in different soil types. The results suggest that different soils have different inherent microbial potential to degrade hydrocarbons, a finding that should be taken into account in impact and risk assessments. Following the development of the microbial community and its recovery is a useful and sensitive way of monitoring the impact and recovery of oil-contaminated soils.
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