Serological relationship and purification of bud necrosis virus, a Tospovirus occurring in peanut (Arachis hypogaea) in India

University of Idaho, Москва, Idaho, United States
Annals of Applied Biology (Impact Factor: 2). 02/2008; 120(2):279 - 286. DOI: 10.1111/j.1744-7348.1992.tb03425.x


SummaryA procedure for the purification of a tospovirus which causes bud necrosis disease (BND) of peanut in India is described. The virus contained three polypeptides of 78 kDa, 54 kDa and 31 kDa. In two ELISA procedures the virus failed to react with antisera to tomato spotted wilt virus (TSWV) obtained from different sources and with an antiserum to impatiens necrotic spot virus (INSV). Additionally, in reciprocal tests TSWV and INSV antigens failed to react with antiserum to the virus infecting peanut in India.In electro-blot immunoassay 54 kDa and 31 kDa polypeptides of the virus reacted with the homologous antiserum. None of the heterologous antisera reacted with any of the three viral polypeptides. On the basis of serological differences the virus that causes BND in India is distinct and therefore has been named bud necrosis virus (BNV). This serotype appears to be restricted to Asia.

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    • "Peanut is affected by several tospoviruses worldwide, including: Tomato spotted wilt virus (TSWV), Groundnut ringspot virus (GRSV), Peanut bud necrosis virus (PBNV), Peanut yellow spot virus (PYSV) and the tentative species Peanut chlorotic fan-spot virus (PCFSV) (Reddy et al., 1992; De Avila et al., 1993; Satyanarayana et al., 1998; Chu et al., 2001). TSWV, the type member, is the most important species. "
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    ABSTRACT: Groundnut ringspot virus (GRSV), genus Tospovirus, is a thrips-transmitted virus infecting peanuts (Arachis hypogaea L.) in Córdoba province, Argentina. Fourteen viral isolates were recovered from Tospovirus-like symptomatic plants from different peanut fields. Viral isolates as GRSV were identified by serological and molecular tests. Nucleotide and derived amino acid sequence analyses of the nucleocapsid (N) gene indicated a high degree of identity between the GRSV peanut isolates, indicating that there is no molecular variability in the N gene of the GRSV that infects peanuts in the cropping area of Córdoba. In this study, we determined the presence of thrips species in the crop, which can potentially transmit the virus. Thrips were observed in all the evaluated peanut fields. Frankliniella schultzei was the most frequently identified species followed by Caliothrips phaseoli and Frankliniella occidentalis. This work reports the presence of F. schultzei and F. occidentalis in peanuts in Argentina for the first time. These results along with the high degree of similarity between the GRSV peanut isolates suggest that the virus could be transmitted by F. schultzei, which has been cited as its most efficient vector.
    Full-text · Article · Jun 2015 · AgriScientia
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    • "The disease was reported from South and South-East Asia, including China (Reddy 1988), and its cause was first identified as TSWV in India (Ghanekar et al. 1979). After characterization, the virus was identified as a distinct Tospovirus and named GBNV (syn., Peanut bud necrosis virus), which is placed in serogroup IV (Reddy et al. 1992; Satyanarayana et al. 1996a). The incidence of groundnut bud necrosis ranges from 5 to 80% in different parts of the Indian subcontinent. "
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    ABSTRACT: Peanut bud necrosis virus (PBNV), genus Tospovirus (family Bunyaviridae), is an important virus infecting peanut and other crops in South India. PBNV isolates naturally infecting groundnut, brinjal, tomato, black gram, field bean, cowpea, cotton, jute, taro and Calotropis plants were collected from different regions of South India and characterized. Infection was confirmed by direct antigen-coating enzyme-linked immunosorbent assay (DAC-ELISA) using PBNV-specific antiserum. The coat protein gene was further amplified using PBNV coat protein-specific primers. The amplicon (830 bp) was cloned and sequenced; sequence analysis revealed that the N gene shared 93–100% and 95–100% sequence identity with PBNV at the nucleotide and amino acid levels, respectively.
    Full-text · Article · Dec 2013 · Journal of Phytopathology
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    • "Calla lily chlorotic spot virus ( Chen et al . 2005 ) . Groundnut bud necrosis virus ( Lakshmi et al . 1995 , Meena et al . 2005 , Reddy et al . 1992 ) . Melon yellow spot virus ( Kato et al . 2000 ) . Watermelon silver mottle virus ( Iwaki et al . 1984 ) ."
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    ABSTRACT: Tospoviruses belong to the sole phytovirus genus, Tospovirus, in the family Bunyaviridae. Tospoviruses are known to be exclusively transmitted by thrips belonging to the family Thripidae and subfamily Thripinae. Of the known 1,710 species of Thripidae only 14 thrips species are currently reported to transmit tospoviruses. Thrips-transmitted tospoviruses cause severe yield losses to several economically important crops in the United States and worldwide. For instance, a single Tospovirus (Tomato spotted wilt virus) alone caused an estimated $1.4 billion in losses in the U.S. over 10 years. Global trade and associated movement of plant materials across borders have introduced tospoviruses and their vectors into newer areas. Advances in serological and molecular techniques have also led to identification of new tospoviruses. This scenario has also initiated new vector-pathogen interactions between introduced and native thrips species and tospoviruses. The goal of this manuscript is to provide a comprehensive and updated list of thrips species that serve as vectors of tospoviruses along with information pertaining to common names, key diagnostic characters, distribution, important crops economically affected, and thrips and Tospovirus-induced symptoms. The manuscript is prepared with special emphasis to the U.S., but information pertaining to other countries is also included.
    Full-text · Article · Mar 2011
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