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Asparagus racemosus Willd (Liliaceae) ameliorates early diabetic nephropathy in STZ induced diabetic rats

Department of Pharmacology, Smt. Kashibai Navale College of Pharmacy, Pune 411 048, India.
Indian journal of experimental biology (Impact Factor: 0.84). 07/2012; 50(7):469-75.
Source: PubMed

ABSTRACT

Diabetic nephropathy is a major "microvascular" complication of diabetes, differs from other causes of chronic kidney diseases in its predictability, with well-defined functional progression from hyperfiltration to micro- to macroalbuminuria to renal failure. The present study was undertaken to investigate the effect of Asparagus racemosus Willd (Liliaceae) on streptozotocin-induced early diabetic nephropathy. Single i.p injection of streptozotocin (55 mg/kg) was administered to induce early diabetic nephropathy in Wistar rats and thereafter treated orally with ethanolic extract of Asparagus racemosus (EEAR) at a dose level of 100 and 250 mg/kg daily for 4 weeks. The efficacy of extract was compared with diabetic control rats. A. racemosus treatment significantly decreased plasma glucose, creatinine, urea nitrogen, total cholesterol and triglyceride levels. Renal hypertrophy, polyuria, hyperfiltration, microalbuminuria and abnormal changes in the renal tissue as well as oxidative stress were effectively attenuated by EEAR treatment. Basement membrane thickening and mesangial proliferation formation without nodules were seen in diabetic rats, whereas these structural changes were reduced in EEAR treated groups. Results of this study suggested that A. racemosus has beneficial effect in the treatment of diabetic

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Available from: D. P Jain, Dec 06, 2014
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    • "Numerous CMs are applied at this point to control this reversible stage of DN [41]. Asparagus racemosus (lu-sun), Radix Astragali (huang-qi), Rosa laevigata (jin-ying-zi), and Piper auritum (hu-jiao) were reported to enhance the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), leading to attenuation of the oxidative stress [5,424344. "
    Jing-Yi Liu · Xiao-Xin Chen · Sydney Chi-Wai Tang · Stephen Cho-Wing Sze · Yi-Bin Feng · Kai-Fai Lee · Kalin Yan-Bo Zhang
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    Preview · Article · Dec 2016 · Chinese Medicine
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    • "It is also recommended for diverse ailments such as neuropathy, nervous disorders and certain infectious diseases (Goyal et al. 2003). AR is reported to possess several pharmacological activities including adaptogenic, antistress , gastroduodenal ulcer protective, anticancer, antiurolithiatic , immunomodulatory and diabetic-induced antinephropathic effects in experimental models (Krishnamurthy et al. 2013; Somania et al. 2012; Sairam et al. 2003). AR has significant amount of steroidal saponins (I–X). "
    [Show abstract] [Hide abstract] ABSTRACT: Asparagus racemosus Linn. (AR) is used worldwide as a medicinal plant. In the present study, the anxiolytic activity of standardized methanolic extract of root of AR (MAR) was evaluated in open-field test (OFT), hole-board, and elevated plus maze (EPM) tests. Rats received oral pretreatment of MAR in the doses of 50, 100, and 200 mg/kg daily for 7 days and then were evaluated for the anxiolytic activity in different animal models. Both MAR (100 and 200 mg/kg) and diazepam (1 mg/kg, p.o.) increased the grooming behavior, number of central squares crossed, and time spent in the central area during OFT. Further, MAR (100 and 200 mg/kg) increased the head-dip and head-dip/sniffing behavior, and decreased sniffing activity in hole-board test. Furthermore, MAR (100 and 200 mg/kg) increased the percentage entries and time spent to open arm in EPM test paradigm. The anxiolytic activity in the experimental models was similar to that of diazepam. MAR (100 and 200 mg/kg) enhanced the level of amygdalar serotonin and norepinephrine. It also increased the expression of 5-HT2A receptors in the amygdala. In another set of experiment, flumazenil attenuated the anxiolytic effect of minimum effective dose of MAR (100 mg/kg) in OFT, hole-board, and EPM tests, indicating GABAA-mediated mechanism. Moreover, the anxiolytic dose of MAR did not show sedative-like effect in OFT and EPM tests compared to diazepam (6 mg/kg, p.o.). Thus, the anxiolytic response of MAR may involve GABA and serotonergic mechanisms. These preclinical data show that AR can be a potential agent for treatment of anxiety disorders.
    Preview · Article · Feb 2014 · Cellular and Molecular Neurobiology
  • [Show abstract] [Hide abstract] ABSTRACT: Diabetes was induced in male adult albino rats by injecting streptozotocin (65 mg/kg b.wt., i.p.) and left untreated for 9 weeks for the development of nephropathy. Animals were treated with methanolic extract of Murraya koenigii (L.) Spreng leaf (MEMK) at two dose levels (200 mg/kg and 400mg/kg) and with standard drug Glimipride (2mg/kg) for 40 days in order to analyse its renoprotective effect, by evaluating metabolic profile, renal function test, electrolyte concentration in blood and urine along with its antioxidant status and histopathological studies. Nephropathy was noted in diabetic rats between 8th-9th week by assessing significant increase in polyuria, proteinuria, serum glucose, albuminuria, creatinine and blood urea nitrogen levels. On 40 days of treatment of MEMK, serum glucose, total cholesterol, HDL- cholesterol, triglyceride including the kidney functions were greatly improved as evidenced by amelioration of blood urea nitrogen, creatinine, serum inulin, total protein concentration, albumin and serum electrolytes (Type IV renal tubular acidosis) and also improvement in antioxidant with decrease in malodialdehyde and increase in catalase, superoxide dismutase and glutathione-s-transferase levels. Histopathological studies of nephropathic animals showed chronic glomerulonephritis with marked improvementin treatment groups. Data from this study suggest that M. koenigii (L.) Spreng leaf possesses a protective effect and attenuates the progression of diabetic nephropathy in animals.
    No preview · Article · Jan 2013 · International Journal of Pharma and Bio Sciences
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