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Lopes-Virella MF, Stone P, Ellis S, Colwell JA. Cholesterol determination in high-density lipoproteins separated by three different methods. Clin Chem 23, 882-884

Clinical Chemistry (Impact Factor: 7.91). 06/1977; 23(5):882-4.
Source: PubMed

ABSTRACT

We describe a simplified method for measuring high-density lipoprotein cholesterol in serum after very-low- and low-density lipoproteins have been precipitated from the specimen with sodium phosphotungstate and Mg2+. Values so obtained correlate well with values obtained with the heparin-Mn2+ precipitation technique (r = 0.95, CV less than 5% in 66% of the subjects studied and between 5 and 10% in the remaining ones) or by ultracentrifugal separation (r = 0.82, CV less than 5% in 80% of the subjects studied and between 5 and 10% in the remaining ones). Our precipitation technique is more appropriate for routine clinical laboratory use.

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Available from: John A Colwell, Feb 19, 2015
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    • "The precipitation was carried out according to the method of LopesVirella et al. (1977)[25]as described in the kit's manufacturer (Randox Laboratories Ltd.) manual. Briefly, 200 í µí¼‡L of plasma was mixed with 500 í µí¼‡L of the precipitant (0.55 mM phosphotungstic acid and 25 mM magnesium chloride) and allowed to sit for 10 min at room temperature. "
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    ABSTRACT: Cold and immobilization stressors can generate oxidative stress as well as skeletal muscle fatigue. Free radicals cause oxidative degradation of lipids, proteins, nucleic acids, and carbohydrates molecules, thereby compromising cell integrity and function. Quail egg had been described as being very functional biochemically, due to the essential biomolecules it contains in very regulated quantity. This study was aimed for evaluating the dietary effect of the egg on lipid profile parameters on selected tissues. The antilipidemic properties of the egg yolk and albumen and poultry (layers) feed were determined in selected tissues in male albino rats assaulted with cold immobilization stress induced on them at 4°C for 2 hours, while diazepam was used as standard antistress drug. Antilipidemic activities were evaluated by lipid profile modulation (HDL, LDL, TRIG., and T-CHOL.). Quantitative and qualitative analyses of fatty acids profile of the yolk hexane-extract were determined by Gas Chromatography and Mass Spectrophotometry (GC-MS). The ameliorative impacts of diazepam (2.5 and 5.0 mg/mL/kg BW), yolk (5 and 10 mL/kg BW), albumen (5 and 10 mL/kg BW), and the feed (5–10 mg/kg BW) were competitively ( p < 0.05 ) specific for each of the tissues. The result of the study suggested yolk and albumen of quail egg and poultry feed as antistress agents as well as lipid modulators.
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    • "HDL-C was measured using Roche/Hitachi HDL-C kit (Cat. number 03030024) and was estimated by phosphotungstic acid precipitation followed by enzymatic analysis in supernatant fraction [53]. LDL-C was calculated by the Friedewald formula after considering its limitations if TG > 400 mg/dL: LDL-C = TC − (HDL-C + TG/5) [54]. "
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    ABSTRACT: Background. Beta 2 -adrenergic receptor (ADRB2) gene polymorphisms, Arg16Gly and Gln27Glu, have been implicated in the pathogenesis of cardiovascular diseases. The aim of this study was to determine the association of these two polymorphisms with the risk of myocardial infarction (MI) in the Egyptian population. Methods. Blood samples were collected from 68 MI patients and 75 healthy controls. They were assessed for the presence of cardiovascular risk factors and genotyped for the Arg16Gly (rs1042713) and Gln27Glu (rs1042714) polymorphisms using allelic-discrimination polymerase chain reaction. Results. There is no significant difference in genotype and allele frequencies at codon 16 between MI patients and controls (íµí±ƒ = 0.919). However, at codon 27, MI risk was higher in Gln 27 homozygous participants than in Glu 27 carriers (íµí±ƒ = 0.045). The haplotype frequency distribution showed significant difference among cases and controls (íµí±ƒ = 0.002); homozygotes for Gly 16 /Gln 27 haplotype were more susceptible to MI than Gly 16 /Glu 27 carriers. Patients with Arg 16 /Gln 27 haplotype had higher serum total cholesterol levels (íµí±ƒ < 0.05) and lower frequency of diabetes in MI patients (íµí±ƒ < 0.01). However, both Glu 27 genotypes and haplotype showed lower frequency of hypertension (íµí±ƒ < 0.001). Conclusions. Our findings suggested that the ADRB2 gene polymorphisms may play an important role in susceptibility of MI among Egyptian population.
    Full-text · Article · Dec 2014
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    • "HDL cholesterol was assessed by precipitation of lipoproteins followed by quantification of HDL in the supernatant. The samples were hydrolyzed with cholesterol esterase and cholesterol oxidase, forming hydrogen peroxide [16]. The hydrogen peroxide formed in the reactions described above was analyzed by performing the horseradish peroxidase reaction in a chromogenic system. "

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