Lopes-Virella MF, Stone P, Ellis S, Colwell JA. Cholesterol determination in high-density lipoproteins separated by three different methods. Clin Chem 23, 882-884

Clinical Chemistry (Impact Factor: 7.91). 06/1977; 23(5):882-4.
Source: PubMed


We describe a simplified method for measuring high-density lipoprotein cholesterol in serum after very-low- and low-density lipoproteins have been precipitated from the specimen with sodium phosphotungstate and Mg2+. Values so obtained correlate well with values obtained with the heparin-Mn2+ precipitation technique (r = 0.95, CV less than 5% in 66% of the subjects studied and between 5 and 10% in the remaining ones) or by ultracentrifugal separation (r = 0.82, CV less than 5% in 80% of the subjects studied and between 5 and 10% in the remaining ones). Our precipitation technique is more appropriate for routine clinical laboratory use.


Available from: John A Colwell, Feb 19, 2015
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    • "Serum glucose was determined according to Trinder (1969) method, serum arginase (Marsch, 1965), serum insulin (Herbert et al., 1965 ), aminotransferase enzyme activities (alanine transaminase (ALT) and aspartate transaminase (AST)) (Reitman and Frankel, 1957), alkaline phosphatase (ALP) activity (Belfield and Goldberg, 1971), c-glutamyl transferase (GGT) activity (Szasz, 1974), total protein (Henry, 1964), total and direct bilirubin levels (Walter and Gerade, 1970), total lipids ((Knight et al., 1972), triglycerides (Fossati and Prencipe, 1982), total cholesterol (Allain et al., 1974 ), high density lipoprotein (HDL) level (Lopez-Virella et al., 1977), and low density lipoprotein (LDH) level (Wieland and Seidel, 1983), liver glucose 6 phosphate dehydrogenase (G6PD) (Kornberg and Horecker, 1955), liver lipid peroxidation (LPO), which was measured by the formation of malondialdehyde (MDA) (Ohkawa et al., 1979), liver reduced glutathione (GSH) (Aykac et al., 1985), liver nitric oxide (NO) (Montgomery and Dymock, 1961 ), liver glutathione-Stransferase (GST) (Habig et al., 1974 ), liver glutathione peroxidase (GPx) (Paglia and Valentine, 1967), liver superoxide dismutase (SOD) (Nishikimi et al., 1972), and liver catalase (CAT) (Aebi, 1984). "
    [Show abstract] [Hide abstract] ABSTRACT: Antioxidant therapy has been thought to be effectual for the prevention and treatment of various diseases including diabetes. Therefore, the present study was designed to investigate the potency of Paracentrotus lividus extract (PLE) for alleviating the complications that resulted after induction of the diabetic rat models (T1DM and T2DM) using high fat diet (HFD)/streptozotocin (STZ). Thirty six male Wistar albino rats were assigned into normal control, T1DM and T2DM untreated, and PLE treated diabetic rat groups. Induction of T1DM was performed by streptozotocin injection (60mg/kg of dissolved in sodium citrate buffer, 0.1mol/L, i.p). T2DM induction through 4weeks of high fat diet (HFD) intervention was followed by a single low dosage of STZ (30mg/kg dissolved in 0.1mol/L citrate buffer at pH 4.5, i.p). Both diabetic rat models showed a significant increase in serum; levels of fasting glucose, total protein, bilirubin, activities of arginase, transaminases (AST and ALT), alkaline phosphatase (ALP), γ glutamyl transferase (GGT), lipid profile parameters, and liver malondialdehyde (MDA). However, T1DM and T2DM rats have decreased levels of serum insulin, and liver glucose 6 phosphate dehydrogenase (G6PD), glutathione reduced (GSH), nitric oxide (NO), and antioxidant enzymes. Furthermore, the present study showed the hypoglycemic, hypolipidemic, and antioxidant potency of the PLE as confirmed by its ability for ameliorating most of the alterations caused in the studied parameters of diabetic rats. In conclusion, PLE may be useful as therapy against oxidative stress and liver damage in both types of diabetes mellitus and is therefore recommended for further studies.
    Full-text · Article · Oct 2016
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    • "Serum was frozen until analysis. Serum total cholesterol, HDL cholesterol, triglycerides, and glucose were determined according to [20,21,22] respectively on the Hitachi 911 automated analyzer using reagent kits. LDL cholesterol was calculated by the Friedewald equation [23]. "
    Full-text · Article · Apr 2016 · Journal of food and nutrition research
    • "Glucose was determined according to Trinder (1969). Plasma cholesterol, LDL-and HDL-cholesterol were determined according to the method of Virella et al. (1977). Triglycerides were determined according to Wahlefeld (1974) . "
    [Show abstract] [Hide abstract] ABSTRACT: The present study aimed to investigate the effects of different levels of dietary supplementation of grape seed extract (GSE) on growth performance, carcass traits and antioxidant status of rabbits under heat stress conditions (temperature humidity index 87.5-93.5). Weaned male New Zealand White (NZW) rabbits about 6 weeks old (n = 144, mean body weight 705 g) were randomly allotted to four dietary groups. The Control group was fed a basal diet without GSE; the experimental groups received the basal diet with 100, 200 and 300 mg GSE/kg (Groups 100 GSE, 200 GSE and 300 GSE, respectively). The experimental period lasted for 8 weeks. Compared with other groups, rabbits of Group 300 GSE had the best body weight gain and feed conversion ratio and the lowest mortality. Dietary GSE improved carcass weight, percentage of hot carcass, intestine and edible giblets, while total non-edible parts were reduced (p ≤ 0.05) in comparison with the Control group. In Groups 200 GSE and 300 GSE, plasma total protein, albumin and globulin were increased (p ≤ 0.05). In contrast, all supplementation levels of GSE reduced (p ≤ 0.05) the plasma concentrations of total lipids, total cholesterol, triglycerides and low-density lipoproteins. Antioxidant enzymes of rabbits (superoxide dismutase, catalase, glutathione peroxidase, glutathione transferase) and total antioxidant capacity in blood were increased (p ≤ 0.05) by adding dietary GSE. However, malondialdehyde was reduced (p ≤ 0.001) with increasing GSE levels. Generally, grape seeds can be considered as rich source of phenolic and flavonoid compounds. The results of the study revealed that all tested levels of GSE were useful as a natural protection against heat stress to maintain performance, carcass traits and antioxidant status and could reduce the negative effects of heat stress in rabbits.
    No preview · Article · Feb 2016 · Archives of animal nutrition
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