Effects of L-arginine immobilization on the anticoagulant activity and hemolytic property of polyethylene terephthalate films

Applied Surface Science (Impact Factor: 2.71). 04/2010; 256(12):3977-3981. DOI: 10.1016/j.apsusc.2010.01.060


Surface modification of polyethylene terephthalate (PET) films was performed with l-arginine (l-Arg) to gain an improved anticoagulant surface. The surface chemistry changes of modified films were characterized by X-ray photoelectron spectroscopy (XPS) and attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. The in vitro anticoagulant activities of the surface-modified PET films were evaluated by blood clotting test, hemolytic test, and the measurement of clotting time including plasma recalcification time (PRT), activated partial thromboplastin time (APTT), and prothrombin time (PT). The data of blood coagulation index (BCI) for l-arginine modified PET films (PET-Arg) was larger than that for PET at the same blood-sample contact time. The hemolysis ratio for PET-Arg was less than that for PET and within the accepted standard for biomaterials. The PRT and APTT for PET-Arg were significantly prolonged by 189 s and 25 s, respectively, compared to those for the unmodified PET. All results suggested that the currently described modification method could be a possible candidate to create antithrombogenic PET surfaces which would be useful for further medical applications.

12 Reads
  • [Show abstract] [Hide abstract]
    ABSTRACT: The impact of NO on the attenuation of thrombotic events and a possible role in ischemia reperfusion injury (IRI) in studies associated with cardiovascular grafts are examined. NO acts primarily through its interactions with soluble guanylyl/guanylate cyclase (sGC), a heterodimeric heme protein leading to cyclic guanosine-3',5'-monophosphate (cGMP), which acts as a second messenger and modulates the cardiovascular protective roles. Hemodynamics has a major role in determining the bioavailability of NO from implanted vascular grafts. Uncoupling of NOS affects the bioavailability of NO as a result of altered production of nitric oxide synthase (NOS) and rapid degradation due to the reactions with free radicals. Nitrites are reduced to NO, and myoglobin has been shown to function as an endogenous nitrite reductase at low oxygen levels. NO concentrations of a functionalized graft can vary when exposed to physiological flow conditions according to the mechanical properties of the vascular graft of concern.
    No preview · Article · Jun 2011 · Chemical Reviews
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Surface modification using plasma processing can significantly change the chemical and physical characteristics of biomaterial surfaces. When used in combination with additional modification techniques such as direct chemical or biochemical methods, it can produce novel biomaterial surfaces, which are anticoagulant, bioactive, and biomimetic in nature. This article reviews recent advances in improving hemocompatibility of biomaterials by plasma surface modification (PSM). The focus of this review is on PSM of the most commonly used polymers for vascular prostheses such as expanded polytetrafluoroethylene (PTFE), polyethylene terephthalate (Dacron(®) ), and next generation of biomaterials, including polyhedral oligomeric silsesquioxane nanocomposite.
    Full-text · Article · Sep 2011 · Biotechnology and Applied Biochemistry
  • [Show abstract] [Hide abstract]
    ABSTRACT: The aim of the present study was to develop and investigate nanoporous activated carbon materials for their ability to adsorb inflammatory cytokines directly from blood, for a range of therapeutic applications, including: systemic inflammatory response syndrome (SIRS) related to sepsis, cardio-pulmonary by-pass surgery, or ischemic reperfusion injury. Building on the previously established relationship between the porous structure of beaded polymer-derived activated carbon and its capacity to adsorb inflammatory molecules, we have developed and characterized monolithic porous carbon columns produced from the same polymer precursor matrix as carbon microbeads. The monolithic columns developed were assessed for their ability to adsorb inflammatory molecules from blood in a circulating system. Preliminary findings demonstrated good removal of the inflammatory cytokines IL-8 (100% removal), IL-6 (80% removal), and TNF (51% removal) from blood. The efficiency of cleansing is dependent on the size of the adsorbed molecule and the porous structure of the monolith, highlighting their potential for use as a hemoadsorption device.
    No preview · Article · Aug 2013 · The International journal of artificial organs
Show more