Current trends in α-helical membrane protein crystallization: An update

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom.
Protein Science (Impact Factor: 2.85). 09/2012; 21(9):1358-65. DOI: 10.1002/pro.2122
Source: PubMed


α-Helical membrane proteins (MPs) are the targets for many pharmaceutical drugs and play important roles in human physiology. In recent years, significant progress has been made in determining their atomic structure using X-ray crystallography. However, a major bottleneck in MP crystallography still remains, namely, the identification of conditions that give crystals that are suitable for structural determination. In 2008, we undertook an analysis of the crystallization conditions for 121 α-helical MPs to design a rationalized sparse matrix crystallization screen, MemGold. We now report an updated analysis that includes a further 133 conditions. The results reveal the current trends in α-helical MP crystallization with notable differences since 2008. The updated information has been used to design new crystallization and additive screens that should prove useful for both initial crystallization scouting and subsequent crystal optimization.

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Available from: Joanne L Parker, Oct 24, 2014
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    • "In contrast, the space between protein units in type II crystals is usually large enough to accommodate the original detergent belt at the expense of stable protein contacts, which likely contributes to the difficulty in obtaining high-quality crystals for diffraction experiments. Among the many detergents available, n-dodecyl-b-D-maltoside (bDM) provides the largest number of successful crystallization trials (Parker and Newstead, 2012). Photosystem II (PSII) is the water-plastoquinone oxidoreductase of oxygenic photosynthesis. "

    Full-text · Dataset · Dec 2014
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    • "The advantages of the statistical design included a comparable success rate to other screens with a smaller number of chemicals, with a more straightforward path towards optimization than a random screen owing to the repetition of specific chemicals within the screen (Tran et al., 2004). More recent examples of this same approach of data mining and creation of screens to encapsulate the results can be found in the Morpheus screen (Gorrec, 2009) and the MemGold screens (Newstead et al., 2008; Parker & Newstead, 2012). 4.4.2. "
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