Article

Bioleaching of heavy metals from a contaminated soil using indigenous Penicillium chrysogenum strain F1

College of Metallurgical Science and Engineering, Central South University, China.
Journal of hazardous materials (Impact Factor: 4.53). 07/2012; 233-234:25-32. DOI: 10.1016/j.jhazmat.2012.06.054
Source: PubMed

ABSTRACT

Bioleaching of heavy metals from contaminated soil using Penicillium chrysogenum strain F1 was investigated. Batch experiments were performed to compare leaching efficiencies of heavy metals between one-step and two-step processes and to determine the transformation of heavy metal fractions before and after bioleaching. The results showed that two-step process had higher leaching efficiencies of heavy metals than one-step process. When the mass ratio of soil to culture medium containing P. chrysogenum strain F1 was 5% (w/v), 50%, 35%, 9% and 40% of Cd, Cu, Pb and Zn were removed in one-step process, respectively. The two-step process had higher removals of 63% Cd, 56% Cu, 14% Pb and 54% Zn as compared with one-step process. The results of the sequential extraction showed that the metals remaining in the soil were mainly bonded in stable fractions after bioleaching. The results of TEM and SEM showed that during bioleaching process, although the mycelium of P. chrysogenum was broken into fragments, no damage was obviously observed on the surface of the living cell except for thinner cell wall, smaller vacuoles and concentrated cytoplasm. The result implied that P. chrysogenum strain F1 can be used to remove heavy metals from polluted soil.

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    • "These disadvantages lead to bioleaching, an alternative remediation technology, receiving wide attention. Many studies have showed that bioleaching remediation has been intensively investigated in the last decade, as it is efficient and potentially cheap compared to other traditional remediation technologies [7] [8] [9] [10]. For the bioleaching technologies, the role of the leaching agent is crucial to remove heavy metals. "
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    ABSTRACT: Bioleaching is an environment-friendly and economical technology to remove heavy metals from contaminated soils. In this study, a biosurfactant-producing strain with capacity of alkaline production was isolated from cafeteria sewer sludge and its capability for removing Zn, Pb, Mn, Cd, Cu, and As was investigated. Phylogenetic analysis using 16S rDNA gene sequences confirmed that the strain belonged to Burkholderia sp. and named as Z-90. The biosurfactant was glycolipid confirmed by thin layer chromatography and Fourier-transform infrared spectroscopy. Z-90 broth was then used for bioleaching remediation of heavy metal-contaminated soils. The removal efficiency was 44.0% for Zn, 32.5% for Pb, 52.2% for Mn, 37.7% for Cd, 24.1% for Cu and 31.6% for As, respectively. Mn, Zn and Cd were more easily removed from soil than Cu, Pb and As, which was attributed to the presence of high acid-soluble fraction of Mn, Zn and Cd and high residual fraction of Cu, Pb and As. The heavy metal removal in soils was contributed to the adhesion of heavy metal-contaminated soil minerals with strain Z-90 and the formation of a metal complex with biosurfactant.
    Full-text · Article · Jan 2016 · Journal of hazardous materials
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    • "The plant growthpromoting bacteria living in soil may be able to reduce susceptibility to heavy metal toxicity (Gupta et al. 2004). Bacteria in the genera Acidithiobacillus, Acetobacter, Acidophilus, Arthrobacter , Pseudomonas and Bacillus are considered to be bioleaching bacteria capable of solubilising heavy metals (Mulligan & Cloutier 2003; Deng et al. 2012). The identification of metaltolerant bacteria is an emerging field of study aimed at overcoming the detrimental effects of heavy metal toxicity on plants. "
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    • "The plant growthpromoting bacteria living in soil may be able to reduce susceptibility to heavy metal toxicity (Gupta et al. 2004). Bacteria in the genera Acidithiobacillus, Acetobacter, Acidophilus, Arthrobacter , Pseudomonas and Bacillus are considered to be bioleaching bacteria capable of solubilising heavy metals (Mulligan & Cloutier 2003; Deng et al. 2012). The identification of metaltolerant bacteria is an emerging field of study aimed at overcoming the detrimental effects of heavy metal toxicity on plants. "
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