Antioxidant and antileukemic properties of selected fenugreek (Trigonella foenum-graecum L.) genotypes grown in western Canada

Department of Biological Sciences, University of Lethbridge, T1K 3M4, Lethbridge, Alberta, Canada
Canadian Journal of Plant Science (Impact Factor: 0.92). 01/2011; 91(1). DOI: 10.4141/cjps10025


2011. Antioxidant and antileukemic properties of selected fenugreek (Trigonella foenum-graecum L.) genotypes grown in western Canada. Can. J. Plant Sci. 91: 99Á105. Fenugreek (Trigonella foenum-graecum L.) is an annual forage legume known to have a number of important medicinal properties such as being anti-diabetic and hyperchloesterolaemic among others. In this study we have investigated the anti-oxidant and antileukemic properties of five fenugreek genotypes (L3068, L3375, Tristar, PI143504 and Amber) grown in western Canada for their potential use as nutraceuticals. Our preliminary experiments conducted in two different laboratories showed that the seeds grown in western Canada have anti-oxidant and antileukemic properties and the genotypes differ in the two traits studied. All the genotypes were found to be good scavengers for hydroxyl and free radicals. Among all the varieties, L3068 showed a higher EC 50 value i.e., lower inhibitory activity for lipid peroxidation than the standard catechin. Although all five extracts showed significant antioxidant activity, the crude extract of Tristar was the most effective. Out of the five cultivars of fenugreek, Amber and L3375 showed a robust antileukemic activity followed by Tristar. Hence we conclude that Tristar has the best potential among all the genotypes tested to be used as a future nutraceutical crop when grown in western Canada.

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Available from: Krishnendu Acharya
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    • "It is one of the oldest medicinal plants known and has long been recognized as a traditional medicine in Asia, Africa and Mediterranean countries (Mebazaa et al., 2009; Naidu et al., 2011). Current research on Fenugreek has shown that it contains active beneficial chemical constituents, including steroidal sapogenins (Taylor et al., 1997), dietary fiber (Naidu et al., 2011), galactomannans (Wu et al., 2009), antioxidants (Naidu et al., 2011), and amino acids such as 4-hydroxyisoleucine which possess anti-diabetic (Kumar et al., 2005), hypocholesterolemic and hypoglycemic properties (Meghwal et al., 2012) which have potential to be used in the treatment of antileukemic (Acharya et al., 2011), antipyretic (Bhatia et al., 2006), antinociceptive, antifertility activity, cure leprosy galactogogue (Bhalke et al., 2009; Khoja et al., 2011), obesity, diabetes and cancer (Thomas et al., 2011). One such active agent is the diosgenin, which inhibits azoxymethane-induced aberrant crypt foci formation in F344 rats and induces apoptosis in HT-29 human colon "
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    ABSTRACT: Trigonella foenum in graecum (Fenugreek) is a traditional herbal plant used to treat disorders like diabetes, high cholesterol, wounds, inflammation, gastrointestinal ailments, and it is believed to have anti-tumor properties, although the mechanisms for the activity remain to be elucidated. In this study, we prepared a methanol extract from Fenugreek whole plants and investigated the mechanism involved in its growth-inhibitory effect on MCF- 7 human breast cancer cells. Apoptosis of MCF-7 cells was evidenced by investigating trypan blue exclusion, TUNEL and Caspase 3, 8, 9, p53, FADD, Bax and Bak by real-time PCR assays inducing activities, in the presence of FME at 65 μg/mL for 24 and 48 hours. FME induced apoptosis was mediated by the death receptor pathway as demonstrated by the increased level of Fas receptor expression after FME treatment. However, such change was found to be absent in Caspase 3, 8, 9, p53, FADD, Bax and Bak, which was confirmed by a time-dependent and dose-dependent manner. In summary, these data demonstrate that at least 90% of FME induced apoptosis in breast cell is mediated by Fas receptor-independently of either FADD, Caspase 8 or 3, as well as p53 interdependently.
    Full-text · Article · Oct 2013 · Asian Pacific journal of cancer prevention: APJCP
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    • "Protection of DNA and other nucleic acids against oxidative damage by the antioxidants present in the fenugreek seeds can prevent, delay or reduce oxidative stress. Acharya et al., 2011 have also reported an inhibition of DNA damage by fenugreek seed extracts 9 . "
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    ABSTRACT: Fenugreek (Trigonella foenum-graecum) seeds were extracted in 80% methanol to examine the content of various potent antioxidant compounds and their influence on in-vitro oxidation of biomolecules i.e. amount. proteins, lipids and DNA. Fenugreek extract had polyphenols (9.47±0.10 mg GAE/g dry seeds) as major antioxidant principle. Gallic acid (170.335µg), caffeic acid (164.550 µg), ellagic acid (184.879 µg) and quercetin (215.814 µg)/g dry weight fenugreek seeds were identified in methanolic extract by HPLC analysis. Other antioxidant compounds ascorbate, tocopherol and riboflavin were present in very low amounts. Antioxidant properties were assayed using DPPH free radical scavenging activity. Fenugreek extract inhibited the metal induced oxidation of proteins and lipids. Presence of the extract could protect DNA against H 2 O 2-induced oxidative stress. IC 50 value of fenugreek seeds was estimated by these mechanisms. The results of this study indicate that polyphenol rich methanolic extract of fenugreek had efficient free radical scavenging, reducing and metal chelating activity to protect biomolecules like proteins, lipids and DNA against oxidative stress.
    Full-text · Article · Jan 2013
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    ABSTRACT: Objective: The objective of present study was to evaluate the effect of trigonelline (TRIG), sitagliptin (SITA) alone and concomitant administration in nicotinamide-streptozotocin induced diabetic nephropathy in Wistar rats. Methods: Diabetes was induced by streptozotocin (65 mg/kg i.p.) injected 15 min after nicotinamide (110 mg/kg, i.p.). Four week later rats were randomly selected and divided into five groups (n=6). The rats were divided into following groups. Group I: non-diabetic. Group II: diabetic control, Group III: diabetic + TRIG (50 mg/kg), Group IV: diabetic + SITA (5 mg/kg), Group V: diabetic + [TRIG (50 mg/kg) + SITA (5 mg/kg)]. The drugs were administered for next 4 weeks starting from 5th week post nicotinamide-STZ injection. Serum glucose (SG) and body weight were measured weekly. Serum creatinine, blood urea nitrogen, serum uric acid, urine volume and creatinine were measured at 0, 4th and 8th week of the study. While superoxide dismutase, reduced glutathione, malondialdehyde and kidney weight measurement and histopathological were carried out at the end of study period. Results: Concomitant administration of TRIG + SITA showed significant decrease in SG, kidney weight, serum creatinine, blood urea nitrogen, serum uric acid, urine volume and the level of malondialdehyde. While significant increase in urine creatinine, activity of superoxide dismutase and glutathione were observed compared to effect of either drug alone. Histopathological study confirmed that TRIG + SITA prevented structural kidney damage. Conclusion: Concomitant administration of trigonelline with sitagliptin prevented development of diabetic nephropathy in rats.
    No preview · Article · Jan 2013 · International Journal of Pharmacy and Pharmaceutical Sciences
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