C-scan photoacoustic microscopy for invivo imaging of Drosophila pupae

Applied Physics Letters (Impact Factor: 3.3). 07/2012; 101(181124). DOI: 10.1063/1.4732797


Quantitative scheme for full-field polarization rotating fluorescence microscopy using a liquid crystal variable retarder Rev. Sci. Instrum. 83, 053705 (2012) The double-helix microscope super-resolves extended biological structures by localizing single blinking molecules in three dimensions with nanoscale precision Appl. Phys. Lett. 100, 153701 (2012) Injector for scattering measurements on fully solvated biospecies Rev. Sci. Instrum. 83, 035108 (2012) Optical-resolution photoacoustic microscopy based on two-dimensional scanning galvanometer Appl. Phys. Lett. 100, 023702 (2012) Simultaneous second-harmonic generation, third-harmonic generation, and four-wave mixing microscopy with single sub-8 fs laser pulses Appl.

Download full-text


Available from: Lei Xi
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The performance of photoacoustic imaging (PAI) is critically dependent on the sensitivity and bandwidth of the transducer. Here, we report the design and fabrication of a polyvinylidene fluoride ultrasonic transducer with variable-thickness layers that demonstrates significantly improved sensitivity and bandwidth. We show that the multilayered transducer provides more than 2-fold increase in sensitivity and more than 65% improvement in bandwidth compared with the conventional single-layer transducer. Photoacoustic imaging of mouse brain with the intact skull and scalp is conducted to demonstrate the power of the multilayered transducer for resolving brain structure in an in vivo setting.
    Full-text · Article · Oct 2012 · Applied Physics Letters
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Photoacoustic microscopy (PAM) is sensitive to optical absorption, while optical coherence tomography (OCT) is based on optical backscattering. Combining PAM and OCT can provide complementary information about biological tissue. Here we present a combined optical-resolution PAM (ORPAM) and OCT system that is integrated through a miniature probe with an overall diameter of 2.3 mm, suitable for insertion through a standard endoscopic or laparoscopic port during minimally invasive surgery or endoscopic exam. The hybrid probe consists of a common optical path for OCT (light delivery/detection) and ORPAM (light excitation) and a 10 MHz unfocused ultrasound transducer for photoacoustic detection. The combined system yields a lateral resolution of 15 μm for both ORPAM and OCT.
    Full-text · Article · Mar 2013 · Applied Optics
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Recently, intensive research has been conducted to accelerate the development of photoacoustic (PA) imaging modality for biomedical applications. The use of acoustic lenses to collect ultrasound signals is of great interest. This Letter presents the design and fabrication of a liquid acoustic diverging lens, which can enlarge the acceptance angle of an ultrasound transducer. This lens possesses an inherent advantage of low acoustic impedance and the convenience to be attached to or detached from a commercial flat transducer. Phantom experiments have been carried out to demonstrate the improvement of using such a liquid lens over using a bare transducer for PA tomography.
    Full-text · Article · Aug 2013 · Optics Letters
Show more