Article

Acaricide resistance status in Indian isolates of Hyalomma anatolicum

Entomology Laboratory, Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, UP, India.
Experimental and Applied Acarology (Impact Factor: 1.62). 07/2012; 58(4). DOI: 10.1007/s10493-012-9592-3
Source: PubMed

ABSTRACT

The multi host tick, Hyalomma anatolicum, is the commonest Hyalomma species in India and cattle serves as the main host of this species. A study to evaluate the acaricide resistance of H. anatolicum to deltamethrin, cypermethrin and diazinon was conducted in 20 areas located in three agro climatic regions known to have abundance of the species. Results obtained by the "larval packet test" (LPT) showed a low grade resistance (level-I, RF <5) in the tick species to both deltamethrin and cypermethrin in 10 areas and higher grade resistance (level-II, RF <25) to deltamethrin in one area, where intensive use of synthetic pyrethroids are practiced for tick control. Low grade resistance to diazinon (level I) was recorded in six areas where organophosphates compounds are extensively used for agricultural practices allowing increased exposure of the moulting instars of the ticks to these chemicals. Biochemical analysis of the samples suggested involvement of esterase and alterations of acetylcholinesterase in the resistance mechanisms.

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    • "The biochemical nature of acaricide resistance involving esterase, glutathione S-transferase, monoxygenase and altered AChE are well documented in R. (B.) microplus (Baxter et al. 1999, Hemingway and Ranson 2000, Jamroz et al. 2000, Villarino et al. 2003). Earlier, significant correlation between resistant factor and α-esterase activity was reported involving ticks collected from wide geographical areas (Shyma et al. 2012, Kumar R. et al. 2013). The PCR amplification of the domain IIIS6 transmembrane segment of the sodium channel gene from the susceptible and resistant isolates showed a clear band at 193 bp. "

    Full-text · Dataset · Jan 2016
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    • "The biochemical nature of acaricide resistance involving esterase, glutathione S-transferase, monoxygenase and altered AChE are well documented in R. (B.) microplus (Baxter et al. 1999, Hemingway and Ranson 2000, Jamroz et al. 2000, Villarino et al. 2003). Earlier, significant correlation between resistant factor and α-esterase activity was reported involving ticks collected from wide geographical areas (Shyma et al. 2012, Kumar R. et al. 2013). The PCR amplification of the domain IIIS6 transmembrane segment of the sodium channel gene from the susceptible and resistant isolates showed a clear band at 193 bp. "

    Full-text · Article · Jan 2016 · The Indian journal of animal sciences
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    • "However, this test can be conducted even with a few ticks available in the field and multiple tests can be carried out with thousands of larvae produced by a few engorged female ticks. Previously, LPT based DCs for deltamethrin, cypermethrin and diazinon were established and successfully validated by Sharma et al. (2012a) and Shyma et al. (2012) for characterization of field tick isolates. As observed in the present study, the bioassay dependent variations in DC have been frequently reported by many workers (Sabatini et al., 2001; Robertson et al., 2006; Castro-Janer et al., 2009) and these may be due to higher level of susceptibility of larvae to these chemicals. "
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    ABSTRACT: Discriminating concentrations (DCs) of malathion, coumaphos, fenvalerate and fipronil were determined to monitor acaricide resistance in field conditions. The LC99 values with 95% confidence interval for malathion, coumaphos, fenvalerate and fipronil were 5126.8 (5011.5-5240.7), 131.0 (120.4-142.5), 2257.5 (2198.1-2318.4) and 6.2 (5.87-6.55), respectively. The narrow confidence intervals in LC50 and LC99 of adult immersion test (AIT) and larval packet test (LPT) affirming the homogeneity of IVRI-I line. Variation in LPT based LC50 and LC99 values of malathion (55.9ppm) and coumpahos (28.4ppm) compared to those obtained in AIT indicating that larvae were more susceptible to these chemicals. The DCs for malathion, coumaphos, fenvalerate and fipronil against adults were determined as 10253.6, 262.0, 4515.0 and 12.4ppm while against larvae the values were 111.8, 56.8, 4014.0 and 9.6ppm, respectively. The working efficiency of DCs was successfully tested in field tick isolates. Establishment of country specific DCs of commonly used insecticides for monitoring of resistance in field ticks is emphasized for establishing tick control strategies. Copyright © 2015 Elsevier GmbH. All rights reserved.
    Full-text · Article · Mar 2015 · Ticks and Tick-borne Diseases
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