Effect of exogenous abscisic acid on the level of antioxidants in Atractylodes macrocephala Koidz under lead stress

MOE Laboratory of Plant-Soil Interaction and College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, China, .
Environmental Science and Pollution Research (Impact Factor: 2.83). 07/2012; 20(3). DOI: 10.1007/s11356-012-1048-0
Source: PubMed


This study hypothesized that the positive or negative effects of exogenous abscisic acid (ABA) on oxidative stress caused by lead were dose dependent. The effects of different levels of ABA (2.5, 5, and 10 mg L(-1)) on lead toxicity in the leaves of Atractylodes macrocephala were studied by investigating plant growth, soluble sugars, proteins, lipid peroxidation, and antioxidative enzymes. Excess Pb inhibited root dry weight, root length, and the number of lateral roots, but increased shoot growth. In addition, lead stress significantly decreased the levels of chlorophyll pigments, protein, and activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and peroxidase (POD). Different levels of ABA significantly increased SOD, CAT, POD, and APX activities, but decreased the level of hydrogen peroxide and malondialdehyde in nonstressed plants. Exogenous application of 2.5 mg L(-1) ABA detoxified the stress-generated damages caused by Pb and also enhanced plant growth, soluble sugars, proteins, and all four antioxidant enzyme activities but reduced Pb uptake of lead-stressed plant compared to lead treatment alone. However, the toxic effects of Pb were further increased by the applications of 5 and 10 mg L(-1) ABA. The levels of antioxidants caused by a low concentration of exogenous ABA might be responsible for minimizing the Pb-induced toxicity in A. macrocephala.

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    • "on MDA. Malondialdehyde (MDA) is a product of peroxidation of unsaturated fatty acids in phospholipids, which may be ascribed to the level of lipid peroxidation[35], and an increase of MDA accumulation as a result of As stress was observed[3]. In the present study, we found that KT application significantly decreased the contents of MDA under As stress (Figure 3(d)), which might be related to a significant increase of SOD and POD activities, and decreased the lipid peroxidation[36]. "
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    ABSTRACT: The effects of different levels of kinetin (KT) application on the growth, biomass, contents of chlorophyll (Chl a, Chl b, and carotenoid), arsenic uptake, and activities of antioxidant enzymes in maize seedlings under arsenic (As) stress were investigated by a hydroponic experiment. The results showed that KT supplementation increased the biomass in terms of root length, root number, fresh weight, and seedling length, and KT treatments also improved the contents of Chl a, As uptake, and Chl a : b ratio compared to cases with As treatment alone. However, no significant changes were observed in carotenoid content, and a reduction was found in Chl b content of seedlings. KT also increased the activities of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) in the leaves of maize seedlings when 0.1 mg/L KT and As were applied, which decreased the content of malondialdehyde (MDA). These results suggested that KT could alleviate the toxicity of As to maize seedlings by keeping the stability of chlorophyll, enhancing the activities of antioxidant enzymes, and inhibiting the lipid peroxidation. In conclusion, the alleviation effect of KT in maize seedlings exposed to As stress was clearly observed in the present study.
    Full-text · Article · Dec 2015 · Advances in Materials Science and Engineering
    • "* Correlation is significant at the 0.05 level, * * Correlation is significant at the 0.01 level, * * * Correlation is significant at the 0.001 level chloroplasts, thus reducing the deleterious effect of metal ions to cells (Wang et al. 2013). (2) The significantly increase in glutathione S-transferase enzyme that transfers glutathione to the toxic compounds and decrease glutathione content may be deduced by its consumption in glutathione S-transferase and in phytochelatin synthesis as a precursor of phytochelatin or in binding with heavy metals (Lin and Aarts 2012). "
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    ABSTRACT: A study was carried out to identify the mechanisms underlying stress caused by Cd and Pb accumulation in leaves of Atriplex halimus L. collected from habitats representing different kinds of pollution. Mean concentrations of Cd and Pb in aerial parts exceeded the critical levels in polluted plants as compared to reference plants. There were significant reduction in guiacol peroxidase, ascorbate peroxidase and glutathione content in most of polluted plants. The results showed increase in superoxide dismutase enzyme in all polluted plants. The significant increment in catalase enzyme, glutathione S-transeferase and ascorbic acid content were observed in most of polluted plants. Results of the nine differential expressed bands showed down regulation of NADH dehydrogenase and Sedoheptulose-bisphosphatase in polluted plants. In contrast, there were six regulated genes in highly polluted plants, representing transcription factors, membrane transporters and ROS detoxification. The transcription level of phytochelatin synthase showed a significant increase in all polluted plants, while heavy metal ATPase transporter expression significantly increased in some polluted plants. In conclusion, A. halimus may use two different strategies against Cd and Pb stress, in which the molecular and physiological features affords similar levels of Cd and Pb tolerance through binding, sequestration and the reduction of harmful effect of heavy metals.
    No preview · Article · Jun 2015 · International Journal of Phytoremediation
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    ABSTRACT: Objective: Atractylodes macrocephala, a famous herbal medicine, is used extensively in the practice of Traditional Chinese Medicine (TCM). Processing procedure is a common approach that usually occurs before A. macrocephala is prescribed. This paper describes a sensitive and specific assay for the determination of principal volatile compounds in crude and processed A. macrocephala. Materials and Methods: The present study concentrated on the development of a static headspace gas chromatography-mass spectrometry (SHS-GC/MS) for separating and identifying of volatile compounds from crude and processed A. macrocephala samples. Results: The results showed that the volatile oil in crude and processed A. macrocephala was markedly quantitatively and qualitatively different. Processing resulted in the reduction of volatile oil contents and variation of chemical compositions in A. macrocephala. Conclusion: The proposed method proved that SHS-GC/MS is rapid and specific, and should also be useful for evaluating the quality of crude and processed medicinal herbs.
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