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Mooreia alkaloidigena gen. nov., sp. nov. and Catalinimonas alkaloidigena gen. nov., sp. nov., alkaloid-producing marine bacteria in the proposed families Mooreiaceae fam. nov. and Catalimonadaceae fam. nov. in the phylum Bacteroidetes

University of California San Diego.
International Journal of Systematic and Evolutionary Microbiology (Impact Factor: 2.51). 06/2012; 63(Pt 4). DOI: 10.1099/ijs.0.043752-0
Source: PubMed

ABSTRACT

Bacterial strains CNX-216T and CNU-914T were isolated from marine sediment samples collected from Palmyra Atoll and off Catalina Island, respectively. Both strains are Gram-negative, aerobic, and produce deep orange to pink colonies and alkaloid secondary metabolites. Cells of strain CNX-216T are short, non-motile rods, whereas cells of strain CNU-914T are short, curved rods with gliding motility. The DNA G+C contents of CNX-216T and CNU-914T are 57.7 and 44.4 mol%, respectively. Strains CNX-216T and CNU-914T contain MK-7 as the predominant menaquinone and iso-C15:0 and C16:1ω5c as the major fatty acids. Phylogenetic analyses reveal that both strains belong to the order Cytophagales in the phylum Bacteroidetes. CNX-216T exhibits low 16S rRNA gene sequence identity (87.1%) with the nearest type strain Cesiribacter roseus 311T (Liu et al., 2012) and forms a well-supported lineage that is outside all currently described families in the order Cytophagales. CNU-914T shares 97.6% sequence identity with 'Porifericola rhodea' strain N5EA6-3A2B (Yoon et al., 2011) and, together with 'Tunicatimonas pelagia' strain N5DB8-4 (Yoon et al., 2012) and four uncharacterized marine bacteria isolated as part of this study, forms a lineage that is clearly distinguished from other families in the order Cytophagales. Based on polyphasic taxonomic characterization, we propose that strains CNX-216T and CNU-914T are novel genera and species for which we propose the names Mooreia alkaloidigena gen. nov., sp. nov., type strain CNX-216T (=DSM 25187T =KCCM 90102T) and Catalinimonas alkaloidigena gen. nov., sp. nov., type strain CNU-914T (=DSM 25186T =KCCM 90101T) within the new families Mooreiaceae fam. nov. and Catalimonadaceae fam. nov.

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