Pseudomonas aeruginosa Bacteriophage PA1O Requires Type IV Pili for Infection and Shows Broad Bactericidal and Biofilm Removal Activities

Department of Microbiology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
Applied and Environmental Microbiology (Impact Factor: 3.67). 06/2012; 78(17):6380-5. DOI: 10.1128/AEM.00648-12
Source: PubMed


We isolated a new lytic Pseudomonas aeruginosa phage that requires type IV pili for infection. PA1Ø has a broad bactericidal spectrum, covering Gram-positive and Gram-negative
bacteria, and can eradicate biofilm cells. PA1Ø may be developed as a therapeutic agent for biofilm-related mixed infections
with P. aeruginosa and Staphylococcus aureus.

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    • "The goal of these studies is to generate broad host range phage that can be used to prevent formation of biofilms or reduce pre-existing biofilms in P. aeruginosa biofilm infections. Numerous studies examined the activity of phage on planktonic P. aeruginosa but relatively few papers have focused on P. aeruginosa biofilms in vitro or in vivo infections associated with biofilms [25,39,40]. In the future we plan to utilize defined cocktails generated as demonstrated here for the treatment of catheter-associated urinary tract infections (CAUTI), where in vivo attempts at phage therapy are nonexistent. "
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