Antifungal activity of coronarin D against Candida albicans. Oral Surg Oral Med Oral Pathol Oral Radiol
Instructor, Department of Oral Diagnosis, Faculty of Dentistry, Naresuan University, Phitsanulok, Thailand.
Oral surgery, oral medicine, oral pathology and oral radiology
07/2012; 114(1):61-6. DOI: 10.1016/j.oooo.2012.01.010
The objective of this study was to investigate the antifungal activity of coronarin D on Candida albicans and its activity was compared with clotrimazole and nystatin.
Coronarin D was extracted by liquid chromatography and used in antifungal testing. The inhibitory effect of coronarin D on C. albicans was determined by cultures and an applied broth dilution test. The rate of fungicidal activity was evaluated by time-kill curves. Morphologic alterations of fungal cells were investigated using scanning electron microscopy.
Coronarin D was effective against C. albicans; the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) were 2 and 4 mg/mL, respectively. The C. albicans killing activity of coronarin D was higher than clotrimazole and nystatin at 2×MFC and 4×MFC, respectively. Morphologic alterations of fungal cells consistent with cell membrane damage were observed in the coronarin D-treated cells.
Coronarin D showed promising antifungal activity against C. albicans in vitro.
Available from: Nitirat Chimnoi
- "Various biological activities of coronarin D were observed, for example, cytotoxic activity against cancer cell [10, 11] and inhibiting both constitutive and inducible nuclear factor-kappa B pathway, a key mediator of inflammation, apoptosis, invasion, and osteoclastogenesis . Recently, antifungal activity of coronarin D against Candida albicans has just been reported . This encouraged coronarin D to be of interest for studying its activity against different microorganisms. "
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ABSTRACT: Coronarin D is a labdane-type diterpene from the rhizomes of Hedychium coronarium. In the view of our ongoing effort to explore its novel biological activity, antimicrobial activity study of coronarin D was performed. The results showed that coronarin D was active against tested Gram-positive bacteria, inactive for tested Gram-negative bacteria, and weakly active against tested fungi. The antibacterial effect of the combination of coronarin D with nine classical antibiotics against four Gram-positive bacteria was also evaluated. The fractional inhibitory concentration indices (FICI) of coronarin D-antibiotics combinations, calculated from the checkerboard assay, were used as synergism indicator. Out of 36 combinations, 47% showed total synergism, 33% had partial synergistic interaction, 17% showed no effect, and 3% showed antagonism. By combination with coronarin D at concentration of 0.25 minimal inhibitory concentration (MIC), the activities of antibiotics were boosted to 4- to 128-fold. These finding suggested an attractive approach to combat the infectious diseases by using coronarin D-antibiotic drug combination.
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This study was conducted to evaluate the antibacterial activity of Garcinia mangostana (GM) extracts on oral pathogens.
The 95% ethanol and 70% acetone extracts of the pericarp of GM was prepared and standardized by determining the amount of α-mangostin, total phenolic compounds and tannins. The antibacterial activity of GM extracts against oral pathogens was investigated by using minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC), and time kill assay. Bacterial morphology was analyzed using scanning electron microscopy (SEM).
The results indicated that the content of α-mangostin, total phenolic compounds and tannins of the both extracts were different. The 95% ethanol extract contained higher α-mangostin and total phenolic compounds. Whereas, the tannins of 70% acetone extract were significantly higher than 95% ethanol extract. The 95% ethanol extract exhibited a potent antibacterial activity with low MIC and MBC values compared to the acetone extract. The morphology of bacteria was significantly changed after treatment with extracts for 24 h. Furthermore, time kill assay revealed that bacterial cells were decreased within 2 h.
GM extracts was effective against oral bacteria pathogens. The antibacterial activity was varied by the different extraction solvents and the distinction in the contents of the compounds among extracts. These findings indicated that GM extracts showed promising antibacterial activity against oral pathogens in vitro.
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