Y chromosome microsatellite isolation from BAC clones in the greater white‐toothed shrew (Crocidura russula)

University of Lausanne, Lausanne, Vaud, Switzerland
Molecular Ecology Notes (Impact Factor: 2.38). 11/2005; 6(1):276 - 279. DOI: 10.1111/j.1471-8286.2005.01180.x


We constructed a microsatellite library from four Crocidura russula Y chromosome-specific bacterial artificial chromosome (BAC) clones. Only one of eight microsatellites was male-specific, despite genome walking to obtain more flanking sequence and testing of 93 primer combinations. Potential reasons for this low success are discussed. The male-specific locus, CRY3, was genotyped in 90 males, including C. russula from across the species range and two related species. The large difference in CRY3 allele size between eastern and western lineages supports earlier reports of high divergence between them. Despite polymorphism of CRY3 in Morocco, only one allele was found throughout the whole of Europe, consistent with previous studies that suggest recent colonization of Europe from a small number of Moroccan founders.

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Available from: Lori Lawson Handley, Apr 15, 2014
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    • "Reduced levels of diversity in sex chromosomes compared with autosomes are widespread ( Ellegren 2009 ) , particularly on the Y chromosome ( Kayser et al . 2003 ; Lawson Handley et al . 2006 ; Frankham 2012 ) . This is attributable to lower recombination rates and effective population sizes ( Kayser et al . 2003 ; Lawson Handley et al . 2006 ; Frankham 2012 ) . In M . fuliginosus , the low effective male population size is due to a female - biased sex ratio ( 3 : 1 : Norbury et al . 1988"
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    • "Finally, this method obviously relies on characterizing informative sex-specific markers, and relative lack of variability on the Y chromosome may be a limiting factor here (Hellborg & Ellegren 2004; Hammond et al. 2006; Lawson Handley et al. 2006a,b). However, the increasing availability of Y-linked markers (Hellborg & Ellegren 2003), particularly microsatellites (Erler et al. 2004; Lawson Handley & Perrin 2006) will hopefully facilitate its future use in this type of study. "
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