A Simple and Safe Volumetric Alternative to the Method of Miles, Misra and Irwin for Counting Viable Bacteria

Journal of Medical Microbiology (Impact Factor: 2.25). 12/1978; 11(4):541-5. DOI: 10.1099/00222615-11-4-541
Source: PubMed


A rapid and simple method for counting viable bacteria is described. The technique involves the use of a semi-automatic micropipette and disposable glass-capillary tubes. Viable counts obtained by this method were not significantly different from those obtained by the Miles, Misra and Irwin method or by a pour-plate method. The micropipette procedure is less hazardous than techniques involving the use of calibrated dropping pipettes.

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