Blood‐derived biomaterials: fibrin sealant, platelet gel and platelet fibrin glue

ISBT Science Series 02/2009; 4(1):136 - 142. DOI: 10.1111/j.1751-2824.2009.01222.x


Blood-derived biomaterials include fibrin sealant (FS) (also called fibrin glue), platelet gel (PG), and platelet fibrin glue. They are used in many surgical fields because of their functional properties and unique physical advantages compared to synthetic products. FS can be made industrially by the fractionation of large plasma pools, or from single plasma donations. Thanks to a high content in fibrinogen, FS exhibits, after activation by thrombin and formation of a strong fibrin clot, tissue sealing and haemostatic properties. PG and platelet fibrin glue are made from single blood donations (platelet concentrates combined or not with cryoprecipitate). Owing to their richness in platelet, PG and PFG can release, upon thrombin activation, a myriad of growth factors that can stimulate cell growth and differentiation, generating much interest for hard and soft tissues regeneration and healing, as well as, increasingly, cell therapy protocols to replace fetal bovine serum. Blood-derived biomaterials have the advantages, over synthetic glues and other biomaterials, of being physiologically compatible with human tissues, and of not inducing tissue necrosis or other tissue reactions. They can be readily colonized by cells and are totally biodegradable in a matter of days to weeks. These blood-derived biomaterials are used increasingly as tissue engineering tools, allowing surgeons to influence and improve the in vitro or in vivo cellular environment to enhance the success of tissue grafting. We review here the three main types of biomaterials that can be made from human blood and describe their biochemical and physiological properties as well as their clinical applications.

Download full-text


Available from: Thierry Burnouf
  • Source
    • "In this study, we applied connection MCCh with fibrin (Fb) in different proportion. Fibrinogen [37] [38] is a soluble plasma glycoprotein synthesized by the liver, which, in the presence of thrombin and calcium ions, is converted into Fb monomers. Fb has been used extensively as a biopolymer scaffold in tissue engineering. "
    [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to describe the mechanical and sorption features of homogeneous and composite membranes which consist of microcrystalline chitosan (MCCh) and fibrin (Fb) in various proportions as well as the in vitro kinetics of platelet-derived growth factor-BB (PDGF-BB) released from ten types of membranes in the presence or absence of amoxicillin (Am). The films were characterized by Fourier transform infrared (FTIR) spectroscopy, mechanical tests: breaking strength (Bs) and elongation at break (Eb), as well as SEM images, and swelling study. The influence of the form of samples (dry or wet) on Young’s modulus ( E ) was also examined. The homogeneous MCCh (M1) and composite M3 and M4 (MCCh : Fb = 2 : 1 and 1 : 1) membranes were characterized by good sorption properties and higher mechanical strength, when compared with Fb (M2) membrane. Connecting MCCh with Fb decreases release of PDGF-BB and increases release of Am. The most efficient release of PDGF-BB was observed in the case of M4 (the optimum MCCh : Fb ratio was 1 : 1) membrane. It was found that the degree of PDGF-BB release from the membrane is influenced by the physicochemical and mechanical characteristics of the films and by its affinity to growth factor PDGF-BB.
    Full-text · Article · Oct 2015 · International Journal of Polymer Science
  • [Show abstract] [Hide abstract]
    ABSTRACT: In missions to India, Pakistan, China, and South Africa, energy efficiency and sustainable energy development have been important focus areas. The missions have provided opportunities to discuss energy efficiency, sustainable energy development and economic progress, and to identify areas where there are opportunities for cooperative ventures in energy efficiency technologies and practices that will lead to both economic and environmental benefits. The format for the development of opportunities in the above countries has been for the Department of Energy (DOE) to work with a broad cross section of industrial companies, energy efficiency organizations and other interested parties to develop country specific teams with designated team leaders for each area identified during the mission. The US teams then draft action plans which serve as a basis for discussion with their international counterparts leading to mutually acceptable implementation plans. US country specific coordinating committees comprising DOE personnel and team leaders plan to hold meetings with their international counterparts to review progress and overcome any issues or barriers encountered. The paper describes the progress made to date with specific examples from the US-China and US-India teams. Also described is the energy efficiency and renewable energy initiative undertaken with Ghana by the Office of Energy Efficiency and Renewable Energy
    No preview · Conference Paper · Sep 1996
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Platelet gels (PG) are new topical single-donor blood products which are attracting great interest in regenerative medicine. They are obtained by mixing a platelet-rich plasma fraction with thrombin to generate a fibrin gel enriched in platelet growth factors (GF). The type of thrombin preparation may affect PG reproducibility. We have determined the impact of 14.6% (v/v) ethanol-stabilized thrombin (EHT) on the release of GF by platelets. Various ratios of EHT and platelet concentrates were mixed to obtain from 2.43 to 7.96% ethanol concentration. Platelet-derived growth factor-AB (PDGF-AB), transforming growth factor-ß1 (TGF-ß1), vascular endothelium growth factor (VEGF), epidermal growth factor (EGF), and insulin-like growth factor-1 (IGF-1) were assessed at 5, 120, and 300 min after PG formation. Protein profiles of thrombin and PG releasates were analyzed by SDS-PAGE. The amount of PDGF-AB, TGF-ß1, and VEGF released per platelet decreased significantly (p < 0.05) with increasing ethanol concentrations but, however, not that of EGF. IGF-1 content was stable, consistent with its presence mostly in plasma. SDS-PAGE indicated that ethanol did not affect fibrin formation. In conclusion, ethanol has a significant impact on the amount of GF released by platelets and should be strictly controlled to standardize PG and optimize clinical benefits.
    Full-text · Article · Jan 2010 · Biologicals
Show more